AB327132

Anti-TRPM4 Antibody [CL11214]

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Mouse Monoclonal TRPM4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TRPM4.

대체 명칭 보기

LTRPC4, TRPM4, Transient receptor potential cation channel subfamily M member 4, hTRPM4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, Melastatin-4, LTrpC-4, LTrpC4

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemistry analysis in human duodenum and pancreas tissues using ab327132 at 1 : 50–1 : 200 dilution. Corresponding TRPM4 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemical staining of human prostate using ab327132 at 1 : 50–1 : 200 dilution, showing moderate membranous positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-TRPM4 Antibody [CL11214] (AB327132)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunofluorescence staining of MCF7 cells using ab327132 at 2–10 µg/ml, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemical staining of human duodenum using ab327132 at 1 : 50–1 : 200 dilution, showing strong membranous positivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemical staining of human kidney using ab327132 at 1 : 50–1 : 200 dilution, showing moderate to strong membranous positivity in cells in tubules.

Supplier Data

Western blot - Anti-TRPM4 Antibody [CL11214] (AB327132)

Western blot analysis in human cell line RT-4 using ab327132 at 1 µg/ml.

All lanes:

Western blot - Anti-TRPM4 Antibody [CL11214] (ab327132)

All lanes:

Human cell line RT-4

Predicted band size: 134.3 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 Antibody [CL11214] (AB327132)

Immunohistochemical staining of human pancreas using ab327132 at 1 : 50–1 : 200 dilution, showing no positivity in exocrine glandular cells as expected.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL11214

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRPM4.

Q8TD43

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium-activated selective cation channel that mediates membrane depolarization (PubMed : 12015988, PubMed : 12842017, PubMed : 29211723, PubMed : 30528822). While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed : 12015988). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane (PubMed : 12015988). It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Plays a role in keratinocyte differentiation (PubMed : 30528822).. Isoform 2. Lacks channel activity.

See full target information TRPM4

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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