AB228724

Anti-Tryptophanyl tRNA synthetase/WRS antibody

Name: Anti-Tryptophanyl tRNA synthetase/WRS antibody
Brand: Abcam Limited
SKU: AB228724
Rating: 4 (1 reviews)

(1 리뷰)

(3 제품이 사용된 논문 )

Rabbit Polyclonal Tryptophanyl tRNA synthetase/WRS antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WARS1.

대체 명칭 보기

IFI53, WARS, WRS, WARS1, Interferon-induced protein 53, Tryptophanyl-tRNA synthetase, IFP53, TrpRS, hWRS

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Paraffin-embedded U87 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Paraffin-embedded C2C12 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for Tryptophanyl tRNA synthetase protein at cytoplasm with ab228724 at a 1/85 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human WARS1. The exact immunogen used to generate this antibody is proprietary information.

P23381

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tryptophanyl tRNA synthetase also known as WRS or TrpRS is an enzyme responsible for attaching tryptophan to its corresponding tRNA during protein synthesis. This enzyme is approximately 53 kDa in mass. It ensures the accurate translation of the genetic code in mRNA into proteins by catalyzing the esterification of tryptophan to the tRNA^Trp. WRS is mostly found in the cytoplasm of cells across various tissues contributing to its role in protein biosynthesis.

Biological function summary

WRS is involved in the important process of protein translation ensuring fidelity in the genetic code transfer from RNA to protein. As part of the aminoacyl-tRNA synthetases family WRS helps in maintaining the overall function and stability of the translational apparatus. It is not typically known to be part of any larger protein complex. In addition to its core function in protein synthesis WRS possesses non-canonical roles related to immune response regulation and angiogenesis.

Pathways

WRS plays key roles in the aminoacyl-tRNA biosynthesis and mTOR signaling pathways. These pathways are essential for regulating protein synthesis and cell growth. In the mTOR pathway WRS's function relates to its interaction with proteins involved in cell growth and proliferation. Its roles within these pathways highlight its influence on cellular homeostasis and protein regulation interacting closely with other synthetases and transcription factors.

WRS has associations with certain cancers and autoimmune disorders. Abnormal expression of WRS is observed in various cancer types reflecting its contribution to dysregulated protein synthesis and cell growth. Additionally WRS has been linked to autoimmune diseases like dermatomyositis where its immune-regulatory roles become compromised. In these conditions WRS's interactions with proteins such as interferons illustrate its importance in the pathophysiology of inflammation and immune response.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction : tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).. Isoform 1. Has no angiostatic activity.. T2-TrpRS. Possesses an angiostatic activity but has no aminoacylation activity (PubMed : 11773625, PubMed : 11773626, PubMed : 14630953). Inhibits fluid shear stress-activated responses of endothelial cells (PubMed : 14630953). Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (PubMed : 14630953).. Isoform 2. Has an angiostatic activity.

See full target information WARS1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomolecules & therapeutics 33:852-865 PubMed40798912

2025

Control of Overly Secreted Tryptophanyl tRNA Synthetase Attenuates Sepsis Severity in a Porcine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Tae Kim,Geunyeol Jeong,Yihyo Kim,HyeonJu Roh,Susung Lim,Jinah Jang,Yun Hui Choi,Kyoung Chul Park,Mirim Jin

EMBO molecular medicine 16:40-63 PubMed38177528

2024

Highly secreted tryptophanyl tRNA synthetase 1 as a potential theranostic target for hypercytokinemic severe sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Tae Kim,Jin Won Huh,Yun Hui Choi,Hee Kyeong Yoon,Tram Tt Nguyen,Eunho Chun,Geunyeol Jeong,Sunyoung Park,Sungwoo Ahn,Won-Kyu Lee,Young-Woock Noh,Kyoung Sun Lee,Hee-Sung Ahn,Cheolju Lee,Sang Min Lee,Kyung Su Kim,Gil Joon Suh,Kyeongman Jeon,Sunghoon Kim,Mirim Jin

Cell discovery 9:125 PubMed38114467

2023

Comprehensive genomic profiling of breast cancers characterizes germline-somatic mutation interactions mediating therapeutic vulnerabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Chen,Cai-Jin Lin,Yu-Chen Pei,Ding Ma,Li Liao,Si-Yuan Li,Lei Fan,Gen-Hong Di,Song-Yang Wu,Xi-Yu Liu,Yun-Jin Wang,Qi Hong,Guo-Liang Zhang,Lin-Lin Xu,Bei-Bei Li,Wei Huang,Jin-Xiu Shi,Yi-Zhou Jiang,Xin Hu,Zhi-Ming Shao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tryptophanyl tRNA synthetase/WRS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise