AB181993

Anti-TSP50 antibody [EPR8675(2)]

RabMAb
Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TSP50 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

CT20, TSP50, PRSS50, Probable threonine protease PRSS50, Cancer/testis antigen 20, Serine protease 50, Testis-specific protease-like protein 50

3 이미지

Flow Cytometry (Intracellular) - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling TSP50with ab181993at a 1/150 dilution, or with a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

Supplier Data

Western blot - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

All lanes:

Western blot - Anti-TSP50 antibody [EPR8675(2)] (ab181993) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43 kDa

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Supplier Data

Western blot - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

All lanes:

Western blot - Anti-TSP50 antibody [EPR8675(2)] (ab181993) at 1/1000 dilution

All lanes:

MDA-MB-435 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa,43 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8675(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TSP50 also known as testis-specific protease 50 is a serine protease with a molecular mass of approximately 53 kDa. This protein is expressed mainly in human testes and has also been observed in certain cancerous tissues. TSP50 shows protease activity which may contribute to its role in cellular processes. While the precise mechanical functions remain not entirely understood TSP50 is involved in the degradation of substrates within cells suggesting a part in protein turnover or processing.

Biological function summary

The action of TSP50 is significant in the regulation of cell growth and differentiation. While not part of a well-defined complex its activity affects cellular homeostasis. The expression pattern of TSP50 mainly restricted to reproductive tissues and some cancer cell types highlights its role in reproductive functions and links to oncogenesis. Its regulation impacts cellular environments possibly promoting proliferation and invasive behavior of cancer cells.

Pathways

TSP50 engages in signaling processes tied to cell cycle regulation and apoptosis. The protein plays a part in pathways related to ubiquitin-dependent proteolysis and might interact with proteins such as p53 a major regulatory protein of the cell cycle and apoptosis. By modulating protein degradation TSP50 can influence cell fate decisions potentially tipping the balance toward growth or death depending on the cellular context.

Increased TSP50 expression associates with certain cancers including breast and prostate cancer. Its expression correlates with malignant progression tumor growth and metastasis. TSP50's interaction with proteins like BRCA1 a known factor in breast cancer risk emphasizes its role in tumor biology. These connections suggest that TSP50 could serve as a potential biomarker for cancer diagnosis or a target for therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May be involved in proteolysis through its threonine endopeptidase activity.

See full target information PRSS50

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

NPJ biofilms and microbiomes 11:93 PubMed40461483

2025

TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Li,Rong Jin,Zhaoxia Wang,Chunxue Niu,Zhenbo Song,Xiaoling Liu,Jian Huang,Huan Zhang,Xia Qian,Feng Gao,Shuyue Wang,Chunlei Yu,Luguo Sun,Yanxin Huang,Lihua Zheng,Guannan Wang,Ying Sun,Xiaoguang Yang,Yongli Bao,Jiawei Li

Zoological research 45:1037-1047 PubMed39147718

2024

PRSS50-mediated inhibition of MKP3/ERK signaling is crucial for meiotic progression and sperm quality.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Xue Niu,Jia-Wei Li,Xiao-Li Li,Lin-Lin Zhang,Yan Lang,Zhen-Bo Song,Chun-Lei Yu,Xiao-Guang Yang,Hai-Feng Zhao,Jia-Ling Sun,Li-Hua Zheng,Xue Wang,Ying Sun,Xiao-Hong Han,Guan-Nan Wang,Yong-Li Bao

Oncology letters 15:9641-9646 PubMed29928339

2018

Cardamonin reduces chemotherapy resistance of colon cancer cells via the TSP50/NF-κB pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Sen Lu,Caizhao Lin,Xiaobin Cheng,Hanju Hua,Tao Xiang,Yu Huang,Xi Huang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TSP50 antibody [EPR8675(2)]

Flow Cytometry (Intracellular) - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Western blot - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Western blot - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise