AB70007

Anti-Tspan-8 antibody

Name: Anti-Tspan-8 antibody
Brand: Abcam Limited
SKU: AB70007
Rating: 4 (1 reviews)

BOND RX™ Validated
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(5 제품이 사용된 논문 )

Rabbit Polyclonal Tspan-8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TSPAN8 aa 50-150.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-8 antibody (AB70007)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-8 antibody (AB70007)

IHC image of ab70007 staining in human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70007, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-Tspan-8 antibody (AB70007)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tspan-8 antibody (AB70007)

Immunofluorescence of HeLa cells treated with ab70007 at 1/500 dilution. Right panel was treated with immunising peptide.

Unknown

Western blot - Anti-Tspan-8 antibody (AB70007)

All lanes:

Western blot - Anti-Tspan-8 antibody (ab70007) at 1/500 dilution

Lane 1:

293 cell extract at 30 µg

Lane 2:

COLO cell extract at 30 µg

Lane 3:

COLO cell extract at 30 µg with immunising peptide

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TSPAN8 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P19075

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tspan-8 also known as Tetraspanin-8 is a protein with a mass of about 27 kDa. This protein belongs to the tetraspanin family characterized by four transmembrane domains. Tspan-8 is expressed in various tissues including the gastrointestinal tract kidney and lung with notable expression in human tumors. It localizes to membranes where it participates in organizing specific microdomains essential for cellular activities like adhesion and migration.

Biological function summary

Tspan-8 impacts several cellular functions such as motility and invasion. It often acts as part of a complex interacting with integrins and other tetraspanins. Through these interactions Tspan-8 facilitates communication between the extracellular matrix and the cytoskeleton. This interaction affects processes like cellular attachment and extracellular matrix remodeling contributing to its functions in normal physiological and pathological conditions.

Pathways

Numerous studies tie Tspan-8 to significant biological pathways particularly those involved in signal transduction and cellular movement. It connects closely with the integrin signaling pathway influencing cellular responses to external signals. In this context Tspan-8 interacts with proteins such as integrin αvβ6 contributing to diverse cellular outcomes. Additionally its role within the epithelial-to-mesenchymal transition (EMT) pathway is relevant for its function in cell plasticity and migration.

Tspan-8 links to cancer and inflammatory conditions. Its overexpression associates with tumor progression and metastasis particularly in cancers like colorectal and pancreatic cancer. The protein's interactions with integrin partners notably integrin αvβ6 could enhance cancer cell invasiveness. Moreover inflammation-related diseases might involve Tspan-8 through its influence on inflammatory cell trafficking and invasion contributing to disease pathology. Understanding these interactions can provide insights into novel therapeutic targets.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 27180357, PubMed : 36078095). Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking (PubMed : 25761241). Promotes ADAM17-mediated TNF processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs) (PubMed : 36078095). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration (PubMed : 25761241). Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR (PubMed : 35904232). Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration (PubMed : 37204469). Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin (PubMed : 37835445).

See full target information TSPAN8

대체 명칭 보기

TM4SF3, TSPAN8, Tetraspanin-8, Tspan-8, Transmembrane 4 superfamily member 3, Tumor-associated antigen CO-029

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell research 32:359-374 PubMed35197608

2022

EGFR signaling promotes nuclear translocation of plasma membrane protein TSPAN8 to enhance tumor progression via STAT3-mediated transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Lu,Liwei An,Guangjian Fan,Lijuan Zang,Weiyi Huang,Junjian Li,Jun Liu,Weiyu Ge,Yuwei Huang,Jingxuan Xu,Shaoqian Du,Yuan Cao,Tianhao Zhou,Huijing Yin,Li Yu,Shi Jiao,Hongxia Wang

Oncogene 40:4884-4893 PubMed34163029

2021

SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junjian Li,Xiaoliang Chen,Liqun Zhu,Zhenghong Lao,Tianhao Zhou,Lijuan Zang,Weiyu Ge,Mengyi Jiang,Jingxuan Xu,Yuan Cao,Shaoqian Du,Yue Yu,Guangjian Fan,Hongxia Wang

Nature communications 10:2863 PubMed31253779

2019

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rongxuan Zhu,Olivier Gires,Liqun Zhu,Jun Liu,Junjian Li,Hao Yang,Gaoda Ju,Jing Huang,Weiyu Ge,Yi Chen,Zhimin Lu,Hongxia Wang

International journal of oncology 52:473-484 PubMed29345284

2018

Quantitative proteomics analysis of the role of tetraspanin-8 in the drug resistance of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Li,Daping Yang,Dejun Cui,Yu Li,Zhao Nie,Jinglin Wang,Li Liang

Oncogene 35:4540-8 PubMed26804173

2016

Therapeutic targeting of tetraspanin8 in epithelial ovarian cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

C S Park,T-K Kim,H G Kim,Y-J Kim,M H Jeoung,W R Lee,N K Go,K Heo,S Lee

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tspan-8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-8 antibody (AB70007)

Immunocytochemistry/ Immunofluorescence - Anti-Tspan-8 antibody (AB70007)

Western blot - Anti-Tspan-8 antibody (AB70007)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise