Anti-TSPEAR antibody [EPR15505] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling TSPEAR using ab192023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain : ab7291 and ab150120 both at 1/500 dilution. The negative controls : Primary ab concentration (anti-TSPEAR) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution. Cells were permeabilized using 0.1% Triton X-100.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSPEAR antibody [EPR15505] (<a href='/ko/products/primary-antibodies/tspear-antibody-epr15505-ab192023'>ab192023</a>) at 1/5000 dilution

All lanes:

human testis tissue lysate at 1/10 dilution

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSPEAR antibody [EPR15505] (<a href='/ko/products/primary-antibodies/tspear-antibody-epr15505-ab192023'>ab192023</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

Predicted band size: 75 kDa

Observed band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSPEAR antibody [EPR15505] (<a href='/ko/products/primary-antibodies/tspear-antibody-epr15505-ab192023'>ab192023</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 80 kDa

false