AB197076

Anti-TTC28/TPRBK antibody - C-terminal

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Rabbit Polyclonal TTC28/TPRBK antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TTC28 aa 2450 to C-terminus.

대체 명칭 보기

KIAA1043, TPRBK, TTC28, Tetratricopeptide repeat protein 28, TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio

2 이미지

Supplier Data

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Detection of TTC28/TPRBK in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197076 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (ab197076)

Predicted band size: 271 kDa

false

Supplier Data

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Lysates prepared wtih NETN lysis buffer.

All lanes:

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (ab197076) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 271 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TTC28 aa 2450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96AY4

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab197076 was affinity purified using an epitope specific to TTC28/TPRBK immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The TTC28 also known as TPRBK is a protein with a molecular weight of approximately 230 kDa. This protein consists of tetratricopeptide repeat domains that are important for protein-protein interactions. TTC28 exhibits a broad expression pattern in human tissues but shows higher expression levels in the brain testis and certain cancerous tissues. Its widespread expression suggests a fundamental role across various biological systems.

Biological function summary

TTC28/TPRBK may influence cellular processes including cell cycle regulation and protein folding. This protein interacts with other proteins as part of a multiprotein complex including components of the cytoskeleton and cell cycle regulators. Its participation in these important processes suggests that TTC28 plays a role in maintaining cellular integrity and function.

Pathways

When considering the cellular mechanism TTC28/TPRBK participates in the cell cycle regulation pathway and possibly impacts cell division. It shows interaction with proteins like cyclins and cyclin-dependent kinases which are central to the progression of the cell cycle. These associations indicate that TTC28's function in pathways reflects its functional importance in regulating proper cellular division and growth.

Researchers associate TTC28/TPRBK with specific types of cancer such as glioblastoma and breast cancer. Aberrant expression or dysfunction of TTC28 may disrupt normal cell cycle progression leading to uncontrolled cell proliferation. Its interaction with oncoproteins such as Cyclin D1 further corroborates its potential role in oncogenesis. Understanding TTC28's involvement in these diseases can contribute to developing targeted therapeutic strategies.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

During mitosis, may be involved in the condensation of spindle midzone microtubules, leading to the formation of midbody.

See full target information TTC28

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2409447121 PubMed39630868

2024

The essential role of TTC28 in maintaining chromosomal stability via HSPA8 chaperone-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Zhang,Meiyi Xiang,Liankun Gu,Jing Zhou,Baozhen Zhang,Wei Tian,Dajun Deng

Neuropsychopharmacology : official publication of 45:1884-1895 PubMed32610340

2020

Activation of tyrosine phosphatase PTP1B in pyramidal neurons impairs endocannabinoid signaling by tyrosine receptor kinase trkB and causes schizophrenia-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohong Qin,Li Zhang,Shelly A Cruz,Alexandre F R Stewart,Hsiao-Huei Chen

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TTC28/TPRBK antibody - C-terminal

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise