AB250803

Anti-TUB3 antibody [EPR13800] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TBA3 antibody. Carrier free. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication.

대체 명칭 보기

YML124C, YM7056.02C, TUB3, Tubulin alpha-3 chain

1 이미지

Supplier Data

Western blot - Anti-TUB3 antibody [EPR13800] - BSA and Azide free (AB250803)

This data was developed using ab184971, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TUB3 antibody [EPR13800] (<a href='/ko/products/primary-antibodies/tub3-antibody-epr13800-ab184971'>ab184971</a>) at 1/50000 dilution

All lanes:

Saccharomyces cerevisiae extract at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

true

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13800

Isotype

IgG

Carrier free

Yes

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

ab250803 is the carrier-free version of ab184971.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TUB3 also known as Tubulin Alpha 3 is a component of microtubules which are cylindrical structures within cells. It has a mass of approximately 50 kDa. TUB3 polymerizes with beta-tubulin to form the tubulin heterodimer which is the building block of microtubules. This protein is expressed in a variety of tissues including the brain and muscles which highlights its importance in these locations.

Biological function summary

TUB3 plays a role in the stabilization of microtubule structures. As a part of the tubulin family it interacts within a larger complex to support cellular dynamics such as shape motility and division. It is essential for processes like intracellular transport where vesicles and organelles rely on microtubules to journey through the cytoplasm. TUB3 also contributes to the proper operation of the mitotic spindle during cell division ensuring accurate chromosome segregation.

Pathways

Its involvement includes the cytoskeleton organization pathway and the mitosis pathway. During cell cycling TUB3 interacts with key proteins like kinesin and dynein which are responsible for motor activities along microtubules. This interaction supports mitotic spindle assembly an important process during the cell replication cycle. TUB3 facilitates various cellular mechanisms tied to structural maintenance and functional operations.

TUB3 has relevancy to neurodegenerative conditions and cancer. Mutations or dysregulation of TUB3 link to Alzheimer's disease where it might affect neuronal transport and stability. In cancer TUB3 expression levels can influence cell proliferation rates. The protein's interplay with many cancer-related kinases such as GSK-3 portrays its potential impact on tumor growth and advancement. Understanding and targeting TUB3 could offer new angles for therapeutic development in these areas.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUB3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TUB3 antibody [EPR13800] - BSA and Azide free

Western blot - Anti-TUB3 antibody [EPR13800] - BSA and Azide free (AB250803)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise