AB140225

Anti-TUBGCP2 antibody [GCP2-01]

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Mouse Monoclonal TUBGCP2 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tubgcp2 aa 1-200.

대체 명칭 보기

Gcp2, Gamma-tubulin complex component 2, GCP-2

1 이미지

Flow Cytometry - Anti-TUBGCP2 antibody [GCP2-01] (AB140225)

Flow Cyt

Unknown

Flow Cytometry - Anti-TUBGCP2 antibody [GCP2-01] (AB140225)

Overlay histogram showing HeLa cells stained with ab140225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140225, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

GCP2-01

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Tubgcp2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q921G8

Specificity

ab140225 recognizes TUBGCP2 and phosphorylated TUBGCP2.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>reducing conditions.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

ab140225 is purified from cell culture supernatant by protein A affinity chromatography to a purity of > 95% (by SDS-PAGE).

보관 버퍼

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle|Store undiluted

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TUBGCP2 also known as Gamma-tubulin complex component 2 plays an important role in the nucleation of microtubules. It has a molecular mass of approximately 95 kDa. The protein is expressed in various tissues throughout the body with significant presence in cells undergoing division. TUBGCP2 belongs to the gamma-tubulin ring complex (γ-TuRC) which is essential for organizing microtubules at the centrosome.

Biological function summary

TUBGCP2 contributes to the formation and stability of the microtubule organizing center (MTOC). TUBGCP2 as part of the γ-TuRC interacts with other components like TUBGCP3 TUBGCP4 and TUBGCP5 creating a scaffold for microtubule attachment. This interaction facilitates the recruitment of tubulin and ensures accurate cell division which is necessary for proper chromosome segregation.

Pathways

TUBGCP2 plays an important role in the cell cycle and mitotic spindle formation. During the cell cycle TUBGCP2 together with proteins such as TUBGCP3 and spindle assembly checkpoint proteins ensures the correct alignment and separation of chromosomes. The protein also interacts with cell signaling pathways that control cell proliferation and division playing a role in maintaining genomic stability.

TUBGCP2 is implicated in cancer and neurodegenerative disorders. Altered expression of TUBGCP2 can lead to improper cell division contributing to the development of various cancers. Moreover its interaction with centrosomal proteins like pericentrin links it to neurological conditions where cell division errors and instability are involved highlighting the importance of TUBGCP2 in both oncogenic processes and neuronal health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation (By similarity). The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation (By similarity). Plays a role in neuronal migration (By similarity).

See full target information Tubgcp2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of neuropathology and experimental neurology 74:723-42 PubMed26079448

2015

Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma.

Applications

Unspecified application

Species

Human

Eduarda Dráberová,Luca D'Agostino,Valentina Caracciolo,Vladimíra Sládková,Tetyana Sulimenko,Vadym Sulimenko,Margaryta Sobol,Nicoletta F Maounis,Elias Tzelepis,Eleni Mahera,Leoš Křen,Agustin Legido,Antonio Giordano,Sverre Mörk,Pavel Hozák,Pavel Dráber,Christos D Katsetos

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TUBGCP2 antibody [GCP2-01]

Flow Cytometry - Anti-TUBGCP2 antibody [GCP2-01] (AB140225)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise