AB187008

Anti-Twist (phospho S68) antibody [EP18178]

Name: Anti-Twist (phospho S68) antibody [EP18178]
Brand: Abcam Limited
SKU: AB187008
Rating: 1 (1 reviews)

RabMAb
Recombinant
20ul selling size
각 태그의 뜻

(1 리뷰)

(9 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Twist phospho S68 antibody. Suitable for IP, Dot, WB and reacts with Human samples. Cited in 9 publications.

3 이미지

Supplier Data

Immunoprecipitation - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Twist (phospho S68) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate with ab187008 at 1/60 dilution.

Western blot was performed from the immunoprecipitate using ab187008 at 1/5000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate 10ug (Input).

Lane 2 : ab187008 IP in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab187008 in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Blocking/Dilution buffer : 5% NFDM/TBST.

PMID : 21502402.

All lanes:

Western blot - Anti-Twist (phospho S68) antibody [EP18178] (ab187008) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate at 10 µg

Lane 3:

HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT), then treated with alkaline phosphatase for 1 hour, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 47 kDa

false

Exposure time: 10s

Supplier Data

Dot Blot - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Dot blot analysis of Twist (phospho S68) labeled with ab187008 at 1/1000 dilution.

Lane 1 : Twist (phospho S68) peptide;

Lane 2 : Twist non-phospho peptide.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP18178

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Twist protein also known as Twist1 is a basic helix-loop-helix (bHLH) transcription factor. It has a molecular mass of approximately 21 kDa. Twist is widely expressed in mesodermal tissues during embryonic development including the somites and limb buds. In adults its expression continues in areas involved in tissue regeneration and wound healing.

Biological function summary

Twist plays significant roles in embryogenesis and in wound healing processes. It functions as an important regulator of mesenchymal cell differentiation and is part of the bHLH family of transcription factors which often form dimers to bind specific DNA sequences. Twist modulates the transcription of genes involved in cell movement and survival. It acts as an important player in the epithelial-mesenchymal transition (EMT) a process important for development and cellular dynamics.

Pathways

Twist integrates into multiple biological networks including the Wnt and Notch signaling pathways. It interacts with proteins like β-catenin in the Wnt pathway and Notch receptors impacting cellular proliferation and fate determination. These interactions highlight its role in regulating cellular behavior and influence various processes such as stem cell maintenance and differentiation.

Twist is implicated in cancer and fibrosis. Its overexpression is often observed in aggressive tumors influencing metastasis through its role in EMT. Twist can interact with oncogenic proteins such as MYC in cancer pathways. In fibrosis aberrant Twist activity leads to excessive tissue remodeling and accumulation of connective tissue. Understanding Twist's function and interactions can aid in developing therapeutic strategies targeting these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator : NPAS2-BMAL1 heterodimer (By similarity).

See full target information TWIST1 pS68

대체 명칭 보기

BHLHA38, TWIST, TWIST1, Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록and refine your search

Translational cancer research 14:584-594 PubMed39974382

2025

LncRNA ZEB1-AS1 promotes the proliferation and migration of non-small cell lung cancer by activating epithelial-mesenchymal transition with STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengjin Chen,Yangwei Yao,Jingpeng Gao

Development (Cambridge, England) 149: PubMed35781329

2022

TWIST1 interacts with β/δ-catenins during neural tube development and regulates fate transition in cranial neural crest cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica W Bertol,Shelby Johnston,Rabia Ahmed,Victoria K Xie,Kelsea M Hubka,Lissette Cruz,Larissa Nitschke,Marta Stetsiv,Jeremy P Goering,Paul Nistor,Sally Lowell,Hanne Hoskens,Peter Claes,Seth M Weinberg,Irfan Saadi,Mary C Farach-Carson,Walid D Fakhouri

Cell death discovery 8:2 PubMed35013126

2022

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation.

Applications

Species

Rat

Jingzhang Fan,Shiming Li,Dawei Wang

Cell death & disease 12:349 PubMed33824282

2021

ZEB1-induced LINC01559 expedites cell proliferation, migration and EMT process in gastric cancer through recruiting IGF2BP2 to stabilize ZEB1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Huojian Shen,Hongyi Zhu,Yuanwen Chen,Zhiyong Shen,Weiqing Qiu,Changlin Qian,Jie Zhang

Technology in cancer research & treatment 20:1533033821990007 PubMed33550957

2021

Silencing of Long Non-Coding RNA FGD5-AS1 Inhibits the Progression of Non-Small Cell Lung Cancer by Regulating the miR-493-5p/DDX5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Cui,Peng Luo,Yao Bai,Jiangping Meng

Cancer management and research 12:6707-6717 PubMed32801903

2020

OIP5-AS1/miR-137/ZNF217 Axis Promotes Malignant Behaviors in Epithelial Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Guo,Jiabao Chen,Dong Liu,Lili Liu

Biochemical pharmacology 180:114152 PubMed32679125

2020

α-Linolenic acid inhibits the migration of human triple-negative breast cancer cells by attenuating Twist1 expression and suppressing Twist1-mediated epithelial-mesenchymal transition.

Applications

Species

Human

Shih-Chung Wang,Hai-Lun Sun,Yi-Hsuan Hsu,Shu-Hui Liu,Chong-Kuei Lii,Chia-Han Tsai,Kai-Li Liu,Chin-Shiu Huang,Chien-Chun Li

International journal of oncology 57:858-870 PubMed32582960

2020

GBP1 promotes erlotinib resistance via PGK1‑activated EMT signaling in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Cheng,Lanying Gou,Ting Wei,Jian Zhang

Cell proliferation 53:e12823 PubMed32515533

2020

LncRNA BBOX1-AS1 upregulates HOXC6 expression through miR-361-3p and HuR to drive cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Xu,Baohua Yang,Lifeng Wang,Yunheng Zhu,Xiuxiang Zhu,Ziyin Xia,Zhen Zhao,Ling Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Twist (phospho S68) antibody [EP18178]

Immunoprecipitation - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Western blot - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Dot Blot - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise