AB244263

Anti-TXNDC/TMX antibody

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Rabbit Polyclonal TXNDC/TMX antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TMX1 aa 200 to C-terminus.

대체 명칭 보기

TMX, TXNDC, TXNDC1, PSEC0085, UNQ235/PRO268, TMX1, Thioredoxin-related transmembrane protein 1, Protein disulfide-isomerase TMX1, Thioredoxin domain-containing protein 1, Transmembrane Trx-related protein

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for TXNDC/TMX using ab244263 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for TXNDC/TMX using ab244263 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for TXNDC/TMX using ab244263 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC/TMX antibody (AB244263)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC/TMX antibody (AB244263)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TXNDC/TMX (green) using ab244263 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Formalin-fixed, paraffin-embedded human testis tissue stained for TXNDC/TMX using ab244263 at 1/200 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-TXNDC/TMX antibody (AB244263)

All lanes:

Western blot - Anti-TXNDC/TMX antibody (ab244263) at 0.4 µg/mL

All lanes:

CAPAN-2 cell lysate

Predicted band size: 32 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TMX1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H3N1

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC also known as TMX or thioredoxin domain-containing protein is a protein with enzymatic properties. This protein functions as a disulfide isomerase and is involved in the formation and rearrangement of disulfide bonds within proteins. TXNDC weighs around 38 kDa and localizes primarily in the endoplasmic reticulum (ER). TXNDC facilitates oxidative protein folding by catalyzing thiol-disulfide exchange reactions. Its expression is common in tissues with high protein synthesis demand such as liver and pancreas.

Biological function summary

Thioredoxin domain-containing protein plays a significant role in maintaining cellular redox homeostasis. It forms part of a complex network with other oxidoreductases contributing to oxidative protein folding processes essential for proper protein maturation and function. Beyond protein folding TXNDC participates in the detoxification of reactive oxygen species helping protect cells from oxidative stress. This function supports normal cellular metabolism and survival under potentially damaging conditions.

Pathways

TXNDC is active within the oxidative protein folding pathway which involves the transfer of electrons from substrate proteins to the molecular oxidant. It collaborates closely with protein disulfide isomerase (PDI) in this pathway to ensure proper protein structure and function. Another significant pathway includes its involvement in the unfolded protein response (UPR) a mechanism that manages protein misfolding stress in the ER. TXNDC modulates the UPR by influencing the levels of reactive oxygen species indirectly impacting the function of pathways regulated by proteins such as IRE1 and PERK.

Alterations in TXNDC function have connections to several pathological conditions. One such condition is cancer where imbalances in TXNDC expression levels can contribute to tumor progression and chemoresistance often in coordination with other redox proteins like thioredoxin itself. Additionally improper TXNDC function can relate to neurodegenerative diseases such as Alzheimer's as oxidative stress and protein misfolding are common features. In Alzheimer's TXNDC's involvement in redox regulation and protein homeostasis links it to the broader pathology involving proteins like amyloid-beta and tau.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Thiredoxin domain-containing protein that participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions (PubMed : 11152479, PubMed : 37648867). Acts as a key inhibitor of the alternative triglyceride biosynthesis pathway by inhibiting the activity of TMEM68/DIESL at the endoplasmic reticulum, thereby restricting accumulation of triacylglycerol (PubMed : 37648867). The alternative triglyceride biosynthesis pathway mediates formation of triacylglycerol from diacylglycerol and membrane phospholipids (PubMed : 37648867). Acts as a protein disulfide isomerase by catalyzing formation or reduction of disulfide bonds (PubMed : 22228764, PubMed : 29932915). Specifically mediates formation of disulfide bonds of transmembrane proteins at the endoplasmic reticulum membrane (PubMed : 22228764). Involved in endoplasmic reticulum-associated degradation (ERAD) via its protein disulfide isomerase activity by acting on folding-defective polypeptides at the endoplasmic reticulum membrane (PubMed : 29932915). Acts as a negative regulator of platelet aggregation following secretion in the extracellular space (PubMed : 30425049). Acts as a regulator of endoplasmic reticulum-mitochondria contact sites via its ability to regulate redox signals (PubMed : 27502484, PubMed : 31304984). Regulates endoplasmic reticulum-mitochondria Ca(2+) flux (PubMed : 27502484).

See full target information TMX1

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell communication and signaling : CCS 22:560 PubMed39574183

2024

Exploiting potential molecular compounds for treating testicular seminoma by targeting immune related genes.

Applications

Unspecified application

Species

Unspecified reactive species

Yankang Cui,Xiaodie Zhou,Jing Zhang,Bo Fang,Jingping Ge,Hao Tang,Bianjiang Liu,Haowei He,Feng Xu,Xuejun Shang

BMC genomics 23:363 PubMed35546662

2022

Ewe breed differences in the cervical transcriptome at the follicular phase of a synchronised oestrous cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Abril-Parreño,Kieran G Meade,Anette Kristine Krogenæs,Xavier Druart,Paul Cormican,Sean Fair

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TXNDC/TMX antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC/TMX antibody (AB244263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Western blot - Anti-TXNDC/TMX antibody (AB244263)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise