Anti-TXNDC5 antibody

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TXNDC5 antibody (AB13820)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab13820 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TXNDC5 antibody (AB13820)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC5 antibody (AB13820)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

• WB

Lab

Western blot - Anti-TXNDC5 antibody (AB13820)

False colour image of Western blot : Anti-TXNDC5 antibody staining at 0.1 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab13820 was shown to bind specifically to TXNDC5. A band was observed at 47 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TXNDC5 knockout cell line ab266609 (knockout cell lysate ab263403). To generate this image, wild-type and TXNDC5 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye^® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216778) at 1/20000 dilution.

All lanes:

Western blot - Anti-TXNDC5 antibody (ab13820) at 0.1 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

TXNDC5 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human TXNDC5 knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-txndc5-knockout-hek-293t-cell-line-ab266609'>ab266609</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 48 kDa

Observed band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-TXNDC5 antibody (AB13820)

All lanes:

Western blot - Anti-TXNDC5 antibody (ab13820) at 0.1 µg/mL

Lane 1:

HEK-293 cell lysate at 35 µg

Lane 2:

A549 cell lysate at 35 µg

Lane 3:

HeLa cell lysate at 35 µg

Lane 4:

HepG2 cell lysate at 35 µg

Predicted band size: 48 kDa

false