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AB124954

Anti-TXNRD1 antibody [EPNCIR129]

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(35 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TXNRD1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 35 publications.

대체 명칭 보기

GRIM12, KDRF, TXNRD1, TR, Gene associated with retinoic and interferon-induced mortality 12 protein, KM-102-derived reductase-like factor, Peroxidase TXNRD1, Thioredoxin reductase TR1, GRIM-12, Gene associated with retinoic and IFN-induced mortality 12 protein

9 이미지
Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab124954 at a dilution of 1 in 120 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

Immunohistochemical staining of paraffin embedded human testis with purified ab124954 at a working dilution of 1/150. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

Immunofluorescence staining of HeLa cells with purified ab124954 at a working dilution of 1/150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124954 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

Unpurified ab124954, at 1/100 dilution, staining TXNRD1 in HeLa cells by Immunofluorescence.

Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • IP

Unknown

Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

ab124954 (purified) at 1/40 immunoprecipitating TXNRD1 in 10 μg Jurkat (Lanes 1 and 2, observed at 55 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Predicted band size: 71 kDa

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Western blot - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • WB

Lab

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 71 kDa,83 kDa

Observed band size: 55 kDa

false

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • WB

Lab

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 71 kDa

Observed band size: 55 kDa

false

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • WB

Lab

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution

Lane 1:

rat liver lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 71 kDa

Observed band size: 55 kDa

false

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)
  • WB

Unknown

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

All lanes:

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/1000 dilution

Lane 1:

Mouse liver lysates at 10 µg

Lane 2:

NIH 3T3 lysates at 10 µg

Lane 3:

RAW264.7 lysates at 10 µg

Lane 4:

Human fetal liver lysates at 10 µg

Lane 5:

Rat liver lysates at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 71 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (3)

  • Carrier free

    Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TXNRD1 antibody [EPNCIR129]

  • HRP

    HRP Anti-TXNRD1 antibody [EPNCIR129]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR129

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB151716

Anti-TXNRD1 antibody [EPR10204(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10204(B)] (AB151716)

  • 0
  • 0

보완 제품

Assay Kits

AB83463

Thioredoxin Reductase Assay Kit (Colorimetric)

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

  • 0
  • 0
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB126704

Anti-GCLM antibody [EPR6667]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] (AB126704)

  • 5
  • 2
Primary Antibodies

AB22604

Anti-Glutathione Peroxidase 1 antibody

KO Validated

Western blot - Anti-Glutathione Peroxidase 1 antibody (AB22604)

  • 5
  • 23
Primary Antibodies

AB62352

Anti-Nrf2 antibody [EP1808Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Nrf2 antibody [EP1808Y] (AB62352)

  • 4
  • 34
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>(Heat to 98°C, allow to cool for 10-20 minutes)</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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제품 세부 정보

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin reductase 1 (TXNRD1) also known as TRXR1 is a selenoprotein that plays an important role in maintaining cellular redox balance by catalyzing the reduction of thioredoxin (TRX). It possesses a molecular mass of approximately 55 kDa. TXNRD1 can be found in many tissues displaying a stronger presence in the liver kidney and pancreas indicating its significance in these organs. The enzyme contains a selenocysteine residue at its active site which is essential for its enzymatic activity.
Biological function summary

TXNRD1 functions as an integral part of the thioredoxin system which includes thioredoxin and NADPH. It maintains the redox homeostasis in cells by regenerating oxidized thioredoxin. This action allows thioredoxin to in turn reduce disulfide bonds in various substrate proteins. Furthermore TXNRD1 participates in regulating cellular processes such as DNA synthesis and repair signal transduction and antioxidant defense. It does not work alone but collaborates with various redox-active components to achieve its roles.

Pathways

TXNRD1 participates in the antioxidant defense and redox signaling pathways. Within the antioxidant defense pathway TXNRD1 along with thioredoxin provides resistance to oxidative stress. In the redox signaling pathway the protein interacts with NF-κB signaling processes impacting cell inflammation and apoptosis. These pathways are vital for cellular health and are connected through thioredoxin emphasizing the interconnected nature of TXNRD1-related processes.

TXNRD1 has associations with cancer and neurodegenerative diseases. Elevated levels of TXNRD1 have been observed in various cancers including lung and hepatocellular carcinoma suggesting a role in tumor growth and survival through redox regulation. Additionally TXNRD1 is linked to neurodegenerative diseases such as Alzheimer's where oxidative stress plays a contributory role. In these conditions TXNRD1 interacts closely with other redox proteins like glutathione reductase highlighting its potential as a therapeutic target.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Reduces disulfide protein thioredoxin (Trx) to its dithiol-containing form (PubMed : 8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. A selenocysteine residue at the C-terminal active site is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2) (PubMed : 10849437).. Isoform 1. Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions.. Isoform 4. Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2.. Isoform 5. Enhances the transcriptional activity of the estrogen receptor ESR2 only (PubMed : 15199063). Mediates cell death induced by a combination of interferon-beta and retinoic acid (PubMed : 9774665).
See full target information TXNRD1
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제품이 사용된 논문 (35)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 16:542 PubMed40691131

2025

HIF-1α alleviates ferroptosis in ulcerative colitis by regulation of GPX4.

Applications

Unspecified application

Species

Unspecified reactive species

Weitao Hu,Yanliang Cai,Daxing Cai,Zongchi Chen,Siying Huang,Su Zhang,Huie Zhuang,Taiyong Fang,Xiaoqing Chen

Biological trace element research : PubMed40555955

2025

Supranutritional Selenomethionine but not Selenite Reduces Malignant Cell Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline E Meyer,Maria Schwarz,Felix B Meyer,René Thierbach,Anna P Kipp

Biological research 57:87 PubMed39574138

2024

Depression like-behavior and memory loss induced by methylglyoxal is associated with tryptophan depletion and oxidative stress: a new in vivo model of neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Md Samsuzzaman,Seong-Min Hong,Jae Hyuk Lee,Hyunjun Park,Keun-A Chang,Hyun-Bum Kim,Myoung Gyu Park,Hyeyoon Eo,Myung Sook Oh,Sun Yeou Kim

Antioxidants (Basel, Switzerland) 13: PubMed38671839

2024

Selenium Deficiency Exacerbates Hyperoxia-Induced Lung Injury in Newborn C3H/HeN Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lora C Bailey-Downs,Laura G Sherlock,Michaela N Crossley,Aristides Rivera Negron,Paul T Pierce,Shirley Wang,Hua Zhong,Cynthia Carter,Kathryn Burge,Jeffrey V Eckert,Lynette K Rogers,Peter F Vitiello,Trent E Tipple

Redox biology 72:103148 PubMed38603946

2024

Selenite selectively kills lung fibroblasts to treat bleomycin-induced pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiun-Han Lin,Chen-Chi Liu,Chao-Yu Liu,Tien-Wei Hsu,Yi-Chen Yeh,Chorng-Kuang How,Han-Shui Hsu,Shih-Chieh Hung

Journal of immunology (Baltimore, Md. : 1950) 211:1561-1577 PubMed37756544

2023

Oxidized Low-Density Lipoprotein Accumulation Suppresses Glycolysis and Attenuates the Macrophage Inflammatory Response by Diverting Transcription from the HIF-1α to the Nrf2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth K Y Ting,Pei Yu,Riley Dow,Eric Floro,Hisham Ibrahim,Corey A Scipione,Sharon J Hyduk,Chanele K Polenz,Olga Zaslaver,Peer W F Karmaus,Michael B Fessler,Hannes L Röst,Michael Ohh,Sue Tsai,Daniel A Winer,Minna Woo,Jonathan Rocheleau,Jenny Jongstra-Bilen,Myron I Cybulsky

Phytotherapy research : PTR 37:3913-3925 PubMed37128130

2023

Procyanidin B2 regulates the Sirt1/Nrf2 signaling pathway to improve random-pattern skin flap survival.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Yurun Zhu,Fei Hu,Lue Liu,Guangjie Shen,Qiming Tu

Cancer research 82:3486-3498 PubMed35916672

2022

Selenium Modulates Cancer Cell Response to Pharmacologic Ascorbate.

Applications

Unspecified application

Species

Unspecified reactive species

Connor S R Jankowski,Joshua D Rabinowitz

Bioengineered 13:13829-13848 PubMed35703190

2022

Exosomal circRNA FNDC3B promotes the progression of esophageal squamous cell carcinoma by sponging miR-490-5p and regulating thioredoxin reductase 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Tang,Qingfeng Zhang,Kui Liu,Yun Huang

Environmental toxicology 37:1173-1184 PubMed35092341

2022

Licochalcone E inhibits trxR1 expression, alters Nrf2/STAT6 signal, and induces antitumor effects in vitro against human SH-SY5Y and SK-N-BE(2) neuroblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Jingjing Zhang,Bailing Liu,Jingli Wei
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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