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AB6211

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

Anti-Tyrosine Hydroxylase 항체 - Neuronal Marker

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(5 리뷰들)

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(99 제품이 사용된 논문 )

Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody detecting Tyrosine Hydroxylase in Western Blot, IHC-Fr, ICC/IF. Suitable for Mouse, Rat.

- Over 70 publications
- Trusted since 2000

대체 명칭 보기

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th

4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)
  • IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.

Image courtesy of QBM Cell Sciences, Ottawa, Canada

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)
  • IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1 : 100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)
  • WB

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab6211) at 1/100 dilution

All lanes:

PC12 cell lysates at 6 µg

Predicted band size: 58 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-FoFr, WB, IHC-Fr, ICC/IF

applications

Immunogen

Synthetic Peptide within Rat TH aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P04177

Specificity

<p>Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.</p>

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대체품

Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9
Primary Antibodies

AB220218

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - BSA and Azide free (AB220218)

  • 0
  • 0

보완 제품

Assay Kits

AB185435

Tyrosine Assay Kit (Colorimetric)

Functional Studies - Tyrosine Assay Kit (Colorimetric) (AB185435)

  • 0
  • 0
Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
  • 0
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Primary Antibodies

AB51253

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

  • 5
  • 9
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity 정보

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제품 세부 정보

Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-FoFr, IHC-Fr, WB in mouse samples.

Anti-Tyrosine Hydroxylase antibody (ab6211) has been cited over 77 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Tyrosine Hydroxylase antibody (ab6211) has high sensitivity and specificity.

Anti-Tyrosine Hydroxylase antibody (ab6211) has 4 independent reviews from customers.

Anti-Tyrosine Hydroxylase antibody (ab6211) specifically detects Tyrosine Hydroxylase (UniProt ID: P04177; Molecular weight: 56kDa) and is sold in a 50 µL selling size.

This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
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특성 및 보관 정보

제형
Liquid
Purity
Whole antiserum
보관 버퍼
pH: 7 - 7.4 Constituents: Whole serum
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (PubMed : 30936473).
See full target information Th
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제품이 사용된 논문 (99)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 8:1453 PubMed41068261

2025

HSPA4 restrains transferrin in dopaminergic neurons to attenuate ferroptosis in a Parkinson's disease model.

Applications

Unspecified application

Species

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Tong Gao,Huanhuan Wei,Qianqian Ju,Yongqi Lin,Xiang Yin,Xiaoyu Liu,Jianhong Shen,Qiuhong Ji,Cheng Sun,Lihua Shen

Nature communications 16:7033 PubMed40908286

2025

Identification of Neuritin 1 as a local metabolic regulator of brown adipose tissue.

Applications

Unspecified application

Species

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Manuela Sánchez-Feutrie,Montserrat Romero,Sónia R Veiga,Núria Borràs-Ferré,Nick Berrow,Martina Ràfols,Noemí Giménez,Andrea Rodgers-Furones,Alba Sabaté-Pérez,Ángela Rodríguez Pérez,Luis Rodrigo Cataldo,Hans Burghardt,David Sebastián,Natàlia Plana,Vanessa Hernández,Laura Isabel Alcaide,Óscar Reina,Maria J Monte,José Juan G Marin,Manuel Palacín,Remy Burcelin,Per Antonson,Jan-Ake Gustafsson,Antonio Zorzano

Molecules (Basel, Switzerland) 30: PubMed40430379

2025

Protective Role of Whey Protein Isolate on MPP-Induced Differentiation of SH-SY5Y Cells by Modulating the Nrf2 Antioxidant Pathway.

Applications

Unspecified application

Species

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Panlekha Rungruang,Morakot Sroyraya,Veerawat Sansri

Frontiers in aging neuroscience 17:1503168 PubMed40201545

2025

Whole-brain 3D imaging of dopaminergic neurons and glial cells in the mouse model of Parkinson's disease induced by 6-OHDA.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Wang,Linglong Xiao,Yifeng Shi,Yaping Wu,Xinyuejia Huang,Yang Wu,Yangyang Xu,Lin Bai,Wei Pan,Jie Zhang,Wei Wang

Acta neuropathologica communications 13:59 PubMed40083022

2025

Cranial radiation disrupts dopaminergic signaling and connectivity in the mammalian brain.

Applications

Unspecified application

Species

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Die Zhang,Riya Thomas,Thanh Thai Lam,Ines Veselinovic,David R Grosshans

Vavilovskii zhurnal genetiki i selektsii 28:744-751 PubMed39722665

2024

Substantia nigra alterations in mice modeling Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

I N Rozhkova,S V Okotrub,E Yu Brusentsev,T A Rakhmanova,D A Lebedeva,V S Kozeneva,N A Shavshaeva,N V Khotskin,S Ya Amstislavsky

eLife 13: PubMed39671236

2024

Projection neurons are necessary for the maintenance of the mouse olfactory circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Luis Sánchez-Guardado,Peyman Razavi,Bo Wang,Antuca Callejas-Marín,Carlos Lois

Animal bioscience 38:236-246 PubMed39210791

2024

Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits.

Applications

Unspecified application

Species

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Yang Chen,Tingting Lu,Yingying Dai,Yu Xue,Bohao Zhao,Xinsheng Wu

ACS nano 18:13277-13285 PubMed38728175

2024

Multifunctional and Flexible Neural Probe with Thermally Drawn Fibers for Bidirectional Synaptic Probing in the Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yeji Kim,Yunheum Lee,Jeongeun Yoo,Kum Seok Nam,Woojin Jeon,Seungmin Lee,Seongjun Park

Neuron 112:1978-1996.e6 PubMed38599212

2024

Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Saptarshi Biswas,Sanjid Shahriar,Galina Bachay,Panos Arvanitis,Danny Jamoul,William J Brunken,Dritan Agalliu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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