AB277105

Anti-Ube2B antibody [PCRP-UBE2B-1C7]

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(2 제품이 사용된 논문 )

Mouse Monoclonal UBE2B antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBE2B.

대체 명칭 보기

RAD6B, UBCH1, UBE2B, Ubiquitin-conjugating enzyme E2 B, E2 ubiquitin-conjugating enzyme B, RAD6 homolog B, Ubiquitin carrier protein B, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-protein ligase B, HR6B, hHR6B

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Flow Cytometry - Anti-Ube2B antibody [PCRP-UBE2B-1C7] (AB277105)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ube2B antibody [PCRP-UBE2B-1C7] (AB277105)

Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ube2B using ab277105 at 2 μg / 10⁶ cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Supplier Data

Immunocytochemistry - Anti-Ube2B antibody [PCRP-UBE2B-1C7] (AB277105)

Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ube2B with ab277105 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

Protein Array - Anti-Ube2B antibody [PCRP-UBE2B-1C7] (AB277105)

Protein Array

Unknown

Protein Array - Anti-Ube2B antibody [PCRP-UBE2B-1C7] (AB277105)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277105.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

PCRP-UBE2B-1C7

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Protein Array, Flow Cyt, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human UBE2B.

P63146

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Zebrafish": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from bioreactor concentrate

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ube2B also known as ubiquitin-conjugating enzyme E2 B plays a mechanical role in the ubiquitination process. This protein carries out its function by transferring ubiquitin from an E1 enzyme to target substrates often assisted by an E3 ligase. Ube2B is a smaller protein with a mass of around 22 kDa. It shows expression in a wide range of tissues including testis and brain suggesting tissue-specific roles.

Biological function summary

The activity of Ube2B is central to the ubiquitin-proteasome pathway where it marks proteins for degradation. Ube2B operates as part of the larger ubiquitination cascade and interacts with various E3 ligases such as RNF8 which helps to mediate its target protein selectivity. This highly regulated process ensures proper cellular protein balance and is critical for maintaining cellular homeostasis.

Pathways

Ube2B has important roles in the DNA damage response and cell cycle regulation. The ubiquitin-proteasome system facilitated by Ube2B regulates the turnover of key cell cycle proteins and supports timely progression through cell cycle phases. Ube2B works closely with proteins such as RAD18 during DNA repair processes facilitating the post-replication repair of DNA lesions.

The altered function or expression of Ube2B impacts specific conditions such as cancer and neurodegenerative diseases. In cancer dysregulation can lead to uncontrolled cell proliferation due to abnormal degradation of regulatory proteins. Similarly neurodegenerative disorders involve Ube2B through aggregation and clearance failures of proteins like tau or alpha-synuclein. Ube2B's interaction with these disease processes accentuates its significance in maintaining cellular health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase (PubMed : 16337599, PubMed : 17108083, PubMed : 17130289, PubMed : 1717990, PubMed : 20061386). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed : 20061386). Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1 (PubMed : 16337599). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation (PubMed : 16337599). May play a role in DNA repair (PubMed : 8062904). Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164' (PubMed : 17108083, PubMed : 17130289). In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation (PubMed : 38182926). May be involved in neurite outgrowth (By similarity).

See full target information UBE2B

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Allergologia et immunopathologia 50:33-39 PubMed35257543

2022

TRIM27 suppresses inflammation injuries in pediatric pneumonia by targeting TLR4/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wang,Baoxia Lu,Jia Liu,Yanhong Gu

Bioengineered 12:7470-7480 PubMed34632937

2021

Upregulation of ubiquitin conjugating enzyme E2B (Ube2b) ameliorates neuropathic pain by regulating Kcna2 (potassium voltage-gated channel subfamily A member 2) in primary afferent neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanzhi Peng,Qingqing Zhang,Hao Cheng,Guizhen Yan,Chunli Xing

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ube2B antibody [PCRP-UBE2B-1C7]

Flow Cytometry - Anti-Ube2B antibody [PCRP-UBE2B-1C7] (AB277105)

Immunocytochemistry - Anti-Ube2B antibody [PCRP-UBE2B-1C7] (AB277105)

Protein Array - Anti-Ube2B antibody [PCRP-UBE2B-1C7] (AB277105)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise