AB243830

Anti-UBE3C antibody

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Rabbit Polyclonal UBE3C antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UBE3C aa 550-700.

대체 명칭 보기

KIAA0010, KIAA10, UBE3C, Ubiquitin-protein ligase E3C, HECT-type ubiquitin transferase E3C, Homologous to E6AP carboxyl terminus homologous protein 2, RTA-associated ubiquitin ligase, HectH2, RAUL

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling UBE3C with ab243830 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-UBE3C antibody (AB243830)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling UBE3C using ab243830 at 4 μg/ml (green) in ICC.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling UBE3C with ab243830 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling UBE3C with ab243830 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling UBE3C with ab243830 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human UBE3C aa 550-700. The exact immunogen used to generate this antibody is proprietary information.

Q15386

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

UBE3C also known as ubiquitin protein ligase E3C is an E3 ubiquitin-protein ligase that plays a mechanical role in tagging proteins for degradation. This tagging involves attaching ubiquitin molecules to substrate proteins which targets them for proteasomal degradation. UBE3C has a molecular mass of approximately 90 kDa. Expression of this protein occurs in various tissues with higher levels found in the brain and testis suggesting these organs may rely more on its function.

Biological function summary

UBE3C regulates protein homeostasis by working as part of the ubiquitin-proteasome system which maintains cellular function by removing damaged or unneeded proteins. It assists in controlling protein quality and stability. Although not typically part of larger multiprotein complexes UBE3C influences the turnover of several cellular proteins. Its action affects many processes including neuronal function and response to cellular stress.

Pathways

UBE3C activity intersects with the ubiquitin-proteasome pathway a fundamental mechanism for protein degradation and turnover in cells. This pathway ensures cellular protein quality and prevents toxic protein accumulation. It has associations with proteins like UCHL1 which also have roles in the ubiquitination process. Moreover UBE3C links to the Wnt signaling pathway by modulating the stability of pathway components such as β-catenin which influences cell proliferation and differentiation.

UBE3C involvement appears significant in neurodegenerative diseases and certain cancers. In neurodegenerative conditions like Alzheimer's disease altered UBE3C expression or function might impact protein aggregates formation similar to its interaction with proteins like tau. In cancer abnormal UBE3C levels relate to changes in protein degradation that can lead to unchecked cell growth and survival affecting proteins like p53 an important tumor suppressor.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains (PubMed : 11278995, PubMed : 12692129, PubMed : 16341092, PubMed : 16601690, PubMed : 24158444, PubMed : 24811749, PubMed : 25752573, PubMed : 25752577, PubMed : 32039437, PubMed : 33637724, PubMed : 34239127). Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 32039437, PubMed : 9575161). Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome (PubMed : 24158444, PubMed : 28396413, PubMed : 31375563). Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins (PubMed : 24811749, PubMed : 31375563). Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation (PubMed : 33637724). Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains; with some preference for 'Lys-48' linkages (PubMed : 11278995, PubMed : 16601690, PubMed : 25752577). Acts as a negative regulator of type I interferon by mediating 'Lys-48'-linked ubiquitination of IRF3 and IRF7, leading to their degradation by the proteasome (PubMed : 21167755). Catalyzes ubiquitination and degradation of CAND2 (PubMed : 12692129).

See full target information UBE3C

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of molecular histology 56:144 PubMed40285932

2025

Identification of the oncogenic role of centromere protein M in non-small cell lung cancer via CDC20/MYBL2/Wnt signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wu,Jun Li,Haoyu Wang,Xu Chang,Qinglong Kong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBE3C antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

Immunocytochemistry - Anti-UBE3C antibody (AB243830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3C antibody (AB243830)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise