JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB121927

Anti-UBE3D antibody

5

(1 리뷰)

|

(3 제품이 사용된 논문 )

Rabbit Polyclonal UBE3D antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UBE3D.

대체 명칭 보기

C6orf157, H10BH, UBE2CBP, UBE3D, E3 ubiquitin-protein ligase E3D, HECT-type E3 ubiquitin transferase E3D, UbcH10-binding protein with a HECT-like domain, Ubiquitin-conjugating enzyme E2C-binding protein

5 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3D antibody (AB121927)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3D antibody (AB121927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling UBE3D with ab121927 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3D antibody (AB121927)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3D antibody (AB121927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling UBE3D with ab121927 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry - Anti-UBE3D antibody (AB121927)
  • ICC

Supplier Data

Immunocytochemistry - Anti-UBE3D antibody (AB121927)

Immunocytochemistry analysis of RH-30 (human bone marrow metastasis cell line) cells labeling UBE3D with ab121927 at 2 μg/mL. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3D antibody (AB121927)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3D antibody (AB121927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder tissue labelling UBE3D with ab121927 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3D antibody (AB121927)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3D antibody (AB121927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling UBE3D with ab121927 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UBE3D. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6J8

style
left-column

보완 제품

Primary Antibodies

AB52866

Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (AB52866)

  • 5
  • 31
Primary Antibodies

AB131272

Anti-ACTL6A antibody [EPR7443]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL6A antibody [EPR7443] (AB131272)

  • 0
  • 0
Primary Antibodies

AB16287

Anti-SSEA4 antibody [MC813-70]

20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (AB16287)

  • 4
  • 11
Primary Antibodies

AB93689

Anti-SOX2 antibody [SP76]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [SP76] (AB93689)

  • 4
  • 1
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

UBE3D also known as Ubiquitin Protein Ligase E3D is an enzyme with a molecular mass of approximately 100 kDa. This protein plays a role as a part of the ubiquitination process which marks proteins for degradation in the proteasome. UBE3D is expressed in various tissues with significant expression in the brain liver and heart. This distribution suggests it has roles in different physiological contexts possibly related to its targets for ubiquitination.
Biological function summary

UBE3D takes part in cellular protein homeostasis and regulation. It functions as an E3 ligase that tags proteins with ubiquitin an activity that usually signals these proteins for breakdown. Although not part of fully characterized complexes UBE3D likely interacts with multiple cofactors that assist in substrate recognition and ubiquitin transfer. This activity helps maintain cellular protein quality and modulate signaling pathways by controlling the abundance of key regulatory proteins.

Pathways

UBE3D is essential in the ubiquitin-proteasome system a pathway important for controlling protein turnover and degradation. This pathway ensures that damaged or misfolded proteins do not accumulate in cells thereby maintaining cellular health. UBE3D is also connected to the p53 signaling pathway through its role in regulating proteins that interact with p53 an important protein involved in cell cycle regulation and apoptosis. UBE3D therefore contributes to cell cycle control and stress responses affecting how cells react to various stimuli.

UBE3D has links to neurological conditions including neurodegenerative disorders. Its dysfunction can affect protein degradation pathways leading to the buildup of proteins that could contribute to diseases such as Alzheimer's and Parkinson's. UBE3D's relation to the p53 pathway also connects it to cancer where it might influence cancer progression through its effects on protein degradation and cell cycle control. Understanding these connections could offer insights into therapeutic targets for these diseases.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome (PubMed : 15749827). Independently of its E3 ubiquitin-protein ligase activity, acts as an inhibitor of CPSF3 endonuclease activity by blocking CPSF3 active site (PubMed : 39032490).
See full target information UBE3D
style
left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death discovery 11:99 PubMed40075082

2025

Deficiency of UBE3D in mice leads to severe embryonic abnormalities and disrupts the mRNA of Homeobox genes via CPSF3.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Mi,Lu Yan,Yu Wu,Yufang Zheng

The Journal of clinical investigation 133: PubMed38099500

2023

Active DHEA uptake in the prostate gland correlates with aggressive prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuebin Zhang,Zengming Wang,Shengsong Huang,Dongyin He,Weiwei Yan,Qian Zhuang,Zixian Wang,Chenyang Wang,Qilong Tan,Ziqun Liu,Tao Yang,Ying Liu,Ruobing Ren,Jing Li,William Butler,Huiru Tang,Gong-Hong Wei,Xin Li,Denglong Wu,Zhenfei Li

Clinical science (London, England : 1979) 130:575-86 PubMed26744410

2016

Epigenetic regulation of cyclooxygenase-2 by methylation of c8orf4 in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Iona C Evans,Josephine L Barnes,Ian M Garner,David R Pearce,Toby M Maher,Xu Shiwen,Elisabetta A Renzoni,Athol U Wells,Christopher P Denton,Geoffrey J Laurent,David J Abraham,Robin J McAnulty
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com