AB244287

Anti-UBF1 antibody

Name: Anti-UBF1 antibody
Brand: Abcam Limited
SKU: AB244287
Rating: 5 (2 reviews)

(2 리뷰들)

(8 제품이 사용된 논문 )

Rabbit Polyclonal UBF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UBTF aa 300 to C-terminus.

대체 명칭 보기

UBF, UBF1, UBTF, Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1

11 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Formalin-fixed, paraffin-embedded human skin tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-UBF1 antibody (AB244287)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UBF1 antibody (AB244287)

PFA-fixed, Triton X-100 permeabilized U-2OS (human bone osteosarcoma epithelial cell line) cells stained for UBF1 (green) using ab244287 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Formalin-fixed, paraffin-embedded mouse cerebral cortex tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Formalin-fixed, paraffin-embedded mouse caudate putamen tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Formalin-fixed, paraffin-embedded mouse hippocampus tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-UBF1 antibody (AB244287)

All lanes:

Western blot - Anti-UBF1 antibody (ab244287) at 0.4 µg/mL

All lanes:

Mouse cerebral cortex lysate

Predicted band size: 89 kDa

false

Unknown

Western blot - Anti-UBF1 antibody (AB244287)

All lanes:

Western blot - Anti-UBF1 antibody (ab244287) at 0.4 µg/mL

Lane 1:

U-2OS (human bone osteosarcoma epithelial cell line) cell lysate transfected with control siRNA

Lane 2:

U-2OS cell lysate transfected with siRNA probe 1

Lane 3:

U-2OS cell lysate transfected with siRNA probe 2

Predicted band size: 89 kDa

false

Unknown

Western blot - Anti-UBF1 antibody (AB244287)

All lanes:

Western blot - Anti-UBF1 antibody (ab244287) at 0.4 µg/mL

All lanes:

Human cerebral cortex lysate

Predicted band size: 89 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human UBTF aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17480

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

UBF1 also known as Upstream Binding Factor 1 is a transcription factor with a mass of approximately 97 kDa. It plays a mechanical role in facilitating the transcription of ribosomal RNA (rRNA) by binding to the core promoter element of rRNA genes. This action stimulates the recruitment of RNA polymerase I to the rDNA promoter site. UBF1 expresses in the nucleolus of eukaryotic cells where it functions as part of a nucleolar complex. Its presence is important for the structural organization and function of the nucleolus.

Biological function summary

UBF1 is essential for the regulation of ribosomal biogenesis. It binds to the rDNA enhancer and the rDNA promoter as part of the transcription initiation complex helping to modulate the synthesis of rRNA. UBF1 works synergistically with other nucleolar proteins such as SL1 and TIF-IA. This complex behavior influences cellular growth and proliferation. The interaction of UBF1 acting either alone or in combination with factors like SL1 controls the transcription rate of rRNA impacting the overall protein synthesis capacity of the cell.

Pathways

UBF1 plays a significant role in the cellular growth and ribosome biogenesis pathways due to its fundamental participation in rRNA transcription. It interacts closely with components of the RNA polymerase I transcription machinery. UBF1 also links to the mTOR signaling pathway which is pivotal for cell growth regulation. Interactions with other proteins such as RRN3 help mediate the link between transcriptional regulation by UBF1 and these broader signaling pathways.

UBF1 links to various pathological conditions especially cancer and growth disorders. Aberrations in UBF1 expression or function can lead to unchecked cellular proliferation and tumorigenesis as seen in certain types of cancer. Its connection to the rRNA synthesis pathway implicates it in growth disorders associated with defective ribosome production. Altered interactions between UBF1 and other proteins like p53 play a role in these disease states either by disrupting normal cell cycle control or by enabling the continued presence of harmful mutations.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I (Pol I) through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element and can activate Pol I promoter escape.

See full target information UBTF

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Viruses 17: PubMed40573390

2025

Sub-Nucleolar Trafficking of Hendra Virus Matrix Protein Is Regulated by Ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyue Zhao,Florian A Gomez,Cassandra T David,Christina L Rootes,Cameron R Stewart,Gregory W Moseley,Stephen M Rawlinson

RNA biology 22:1-16 PubMed40126102

2025

Nucleolar ribosomal RNA synthesis continues in differentiating lens fiber cells until abrupt nuclear degradation required for ocular lens transparency.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle Rayêe,U Thomas Meier,Carolina Eliscovich,Aleš Cvekl

Scientific reports 14:14710 PubMed38926592

2024

Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio Peter D'Incal,Elisa Cappuyns,Kaoutar Choukri,Kevin De Man,Kristy Szrama,Anthony Konings,Lina Bastini,Kim Van Meel,Amber Buys,Michele Gabriele,Ludovico Rizzuti,Alessandro Vitriolo,Giuseppe Testa,Fabio Mohn,Marc Bühler,Nathalie Van der Aa,Anke Van Dijck,R Frank Kooy,Wim Vanden Berghe

Journal of cell science 136: PubMed37921359

2023

The antiproliferative effect of FGF2 in K-Ras-driven tumor cells involves modulation of rRNA and the nucleolus.

Applications

Unspecified application

Species

Unspecified reactive species

Francisca N de Luna Vitorino,Michaella J Levy,Rosangela A Mansano Wailemann,Mariana Lopes,Mariana Loterio Silva,Mihaela E Sardiu,Benjamin A Garcia,Maria Cristina Machado Motta,Carla Columbano Oliveira,Hugo Aguirre Armelin,Laurence A Florens,Michael P Washburn,Julia Pinheiro Chagas da Cunha

British journal of cancer 128:766-782 PubMed36526675

2022

STAT3 potentiates RNA polymerase I-directed transcription and tumor growth by activating RPA34 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Zhang,Juan Wang,Xiaoye Song,Deen Yu,Baoqiang Guo,Yaoyu Pang,Xiaomei Yin,Shasha Zhao,Huan Deng,Shihua Zhang,Wensheng Deng

Human molecular genetics 32:104-121 PubMed35925837

2022

TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wang,Qiyue Chen,Xin Wang,Shasha Zhao,Huan Deng,Baoqiang Guo,Cheng Zhang,Xiaoye Song,Wensheng Deng,Tongcun Zhang,Hongwei Ni

Genes 12: PubMed34573302

2021

Evolution, Expression and Meiotic Behavior of Genes Involved in Chromosome Segregation of Monotremes.

Applications

Unspecified application

Species

Unspecified reactive species

Filip Pajpach,Linda Shearwin-Whyatt,Frank Grützner

International journal of sport nutrition and exercise metabolism 31:209-216 PubMed33601335

2021

Daily Myofibrillar Protein Synthesis Rates in Response to Low- and High-Frequency Resistance Exercise Training in Healthy, Young Men.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon J Shad,Janice L Thompson,James Mckendry,Andrew M Holwerda,Yasir S Elhassan,Leigh Breen,Luc J C van Loon,Gareth A Wallis

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Immunocytochemistry/ Immunofluorescence - Anti-UBF1 antibody (AB244287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

Western blot - Anti-UBF1 antibody (AB244287)

Western blot - Anti-UBF1 antibody (AB244287)

Western blot - Anti-UBF1 antibody (AB244287)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (8)

Product promise