AB223746

Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody

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Rabbit Polyclonal Ubiquinol-Cytochrome C Reductase Core Protein I antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UQCRC1 aa 100-300.

대체 명칭 보기

Complex III subunit 1, Core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1, UQCRC1

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Paraffin-embedded human lymph node tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Paraffin-embedded human small intestine tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Paraffin-embedded human heart muscle tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

All lanes:

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (ab223746) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

All lanes:

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (ab223746) at 1/100 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 53 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UQCRC1 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

P31930

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquinol-Cytochrome C Reductase Core Protein I also known as Core Protein I of the cytochrome c reductase complex functions as part of the mitochondrial electron transport chain. It has a molecular weight of around 52 kDa. The protein plays an integral role within the inner mitochondrial membrane primarily performing mechanical roles in electron transfer. Ubiquinol-Cytochrome C Reductase Core Protein I also goes by its abbreviated symbol UQCRC1 and is expressed broadly across tissues with high energy demands.

Biological function summary

UQCRC1 acts as a core component of Complex III also referred to as Cytochrome bc1 complex. This complex sits centrally within the mitochondrial respiratory chain catalyzing the transfer of electrons from ubiquinol to cytochrome c. By facilitating this electron transfer UQCRC1 aids in generating a proton gradient across the inner mitochondrial membrane essential for ATP synthesis during oxidative phosphorylation.

Pathways

UQCRC1 finds itself prominently embedded in the oxidative phosphorylation and electron transport chain pathways. It directly interacts with other proteins such as cytochrome c and cytochrome reductase emphasizing its cooperative function in maintaining cellular energy balance. The seamless integration of UQCRC1 into these pathways highlights its importance in sustaining efficient mitochondrial operation a critical element for cellular respiration.

UQCRC1 shows connections to conditions involving mitochondrial dysfunction such as Leigh syndrome and mitochondrial myopathy. These disorders sometimes exhibit impaired electron transport impacting ATP production. Proteins like cytochrome c and other cytochrome reductase components often participate in these disorder pathways indicating potential targets for therapeutic interventions that aim to restore or augment mitochondrial function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). Seems to play an important role in the maintenance of proper mitochondrial function in nigral dopaminergic neurons (PubMed : 33141179).

See full target information UQCRC1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature : PubMed38057666

2023

A human embryonic limb cell atlas resolved in space and time.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Zhang,Peng He,John E G Lawrence,Shuaiyu Wang,Elizabeth Tuck,Brian A Williams,Kenny Roberts,Vitalii Kleshchevnikov,Lira Mamanova,Liam Bolt,Krzysztof Polanski,Tong Li,Rasa Elmentaite,Eirini S Fasouli,Martin Prete,Xiaoling He,Nadav Yayon,Yixi Fu,Hao Yang,Chen Liang,Hui Zhang,Raphael Blain,Alain Chedotal,David R FitzPatrick,Helen Firth,Andrew Dean,Omer Ali Bayraktar,John C Marioni,Roger A Barker,Mekayla A Storer,Barbara J Wold,Hongbo Zhang,Sarah A Teichmann

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (AB223746)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise