AB236002

Anti-UCH37 antibody [EP4897] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal UCH37 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

UCH37, AD-019, CGI-70, UCHL5, Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH-L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma tissue labelling UCH37 with ab133508 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human colorectal carcinoma. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling UCH37 with ab133508 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse liver. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labelling UCH37 with ab133508 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse liver cancer. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling UCH37 with ab133508 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat liver. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

Supplier Data

Western blot - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-UCH37 antibody [EP4897] (<a href='/ko/products/primary-antibodies/uch37-antibody-ep4897-ab133508'>ab133508</a>) at 1/10000 dilution

Lane 1:

Human colon lysate at 20 µg

Lane 2:

Mouse breast lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Observed band size: 36 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4897

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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제품 세부 정보

ab236002 is the carrier-free version of ab133508.

This product has switched from a hybridoma to recombinant production method on 25th May 2023.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

UCH37 also known as ubiquitin carboxyl-terminal hydrolase L5 (UCHL5) is a deubiquitinating enzyme of approximately 37 kDa mass. This protein functions by cleaving ubiquitin moieties from polyubiquitin chains facilitating protein degradation or recycling processes. UCH37 expression is observed in various tissues with higher expression noted in the liver and kidney indicating its significant cellular roles in these organs.

Biological function summary

UCH37 plays a role in the ubiquitin-proteasome system where it is an integral component of the 19S regulatory particle of the 26S proteasome complex. Through this association UCH37 participates in the removal of ubiquitin from substrates targeted for proteasomal degradation. This action regulates protein turnover and prevents unnecessary degradation of proteins which is important for maintaining cell homeostasis and controlling protein quality.

Pathways

The modulation of proteostasis by UCH37 directly influences the ubiquitin-proteasome pathway and the associated cellular stress responses. Its activity can impact the NF-kappaB signaling pathway by regulating the stability of components within this pathway interacting with other proteins such as the proteasome subunit RPN13 and proteasome-associated DUBs. The precise regulation of protein levels within these pathways ensures that cells respond appropriately to stress and inflammation.

Abnormal regulation or expression of UCH37 contributes to several pathological conditions. Cancer and neurodegenerative disorders are two prominent examples in which UCH37 plays a role. Elevated levels or dysfunctional UCH37 activity can disrupt proteostasis leading to uncontrolled cell proliferation or cell death resistance in cancer or the accumulation of misfolded proteins in conditions like Alzheimer’s disease. Additionally interactions of UCH37 with oncogenic proteins or tau proteins in neurodegenerative conditions reflect its relevance in the progression of these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.

See full target information UCHL5

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in aging neuroscience 12:575481 PubMed33328957

2020

Alpha-Synuclein Accumulation and Its Phosphorylation in the Enteric Nervous System of Patients Without Neurodegeneration: An Explorative Study.

Applications

Unspecified application

Species

Unspecified reactive species

Lu-Lu Bu,Kai-Xun Huang,De-Zhi Zheng,Dan-Yu Lin,Ying Chen,Xiu-Na Jing,Yan-Ran Liang,En-Xiang Tao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UCH37 antibody [EP4897] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

Western blot - Anti-UCH37 antibody [EP4897] - BSA and Azide free (AB236002)

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise