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AB248168

Anti-UCKL1 antibody [EPR7384] - BSA and Azide free

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Rabbit Recombinant Monoclonal UCKL1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

URKL1, F538, UCKL1, Uridine-cytidine kinase-like 1

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] - BSA and Azide free (AB248168)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] - BSA and Azide free (AB248168)

This data was developed using ab126754, the same antibody clone in a different buffer formulation.

ab126754, at 1/100 dilution, staining UCKL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-UCKL1 antibody [EPR7384] - BSA and Azide free (AB248168)
  • WB

Unknown

Western blot - Anti-UCKL1 antibody [EPR7384] - BSA and Azide free (AB248168)

This data was developed using ab126754, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UCKL1 antibody [EPR7384] (<a href='/ko/products/primary-antibodies/uckl1-antibody-epr7384-ab126754'>ab126754</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

false

OI-RD Scanning - Anti-UCKL1 antibody [EPR7384] - BSA and Azide free (AB248168)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-UCKL1 antibody [EPR7384] - BSA and Azide free (AB248168)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7384

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB308252

Anti-UCK2 antibody [EPR27020-82]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-UCK2 antibody [EPR27020-82] (AB308252)

  • 0
  • 0
Primary Antibodies

AB122193

Anti-GLT25D2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLT25D2 antibody (AB122193)

  • 0
  • 0
Primary Antibodies

AB80053

Anti-SEC31B antibody

  • 0
  • 0
Primary Antibodies

AB194752

Anti-Stathmin 1 (phospho S25) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S25) antibody (AB194752)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

ab248168 is the carrier-free version of ab126754.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Uridine-cytidine kinase-like 1 (UCKL1) also known as FLJ10540 is an enzyme with a molecular mass of approximately 38 kDa. It catalyzes the phosphorylation of uridine and cytidine to their corresponding monophosphate forms. UCKL1 localizes in the cytoplasm and nucleus of cells and expresses highly in testis tissues but also appears in liver pancreas and certain tumor cells. Its ability to phosphorylate nucleosides makes it an important player in nucleotide metabolism.
Biological function summary

UCKL1 plays a significant role in cellular nucleotide synthesis. It supports the salvage pathways which provide an efficient route for nucleotide production. This protein is not part of a larger complex but interacts with other nucleotide synthesis enzymes to maintain adequate levels of nucleotides in cells. Through these interactions it helps regulate cellular proliferation by ensuring adequate nucleotide supply for DNA and RNA synthesis.

Pathways

UCKL1 is involved in nucleotide metabolism with particular importance in the pyrimidine salvage pathway. It works closely with other kinases and enzymes like nucleoside diphosphate kinases which further phosphorylate the monophosphates. This salvage pathway complements the de novo synthesis of nucleotides ensuring rapid cell proliferation required during cell growth and repair.

UCKL1 has associations with cancer development and progression particularly in hepatocellular carcinoma. Its overexpression links to increased cellular proliferation and tumor progression. UCKL1 may intersect with proteins like p53 influencing cancer cell survival pathways. Additionally high levels of UCKL1 correlate with autoimmune disorders although the precise mechanism is not fully understood. The binding interactions of UCKL1 with pathological proteins require further research to clarify these disease connections.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May contribute to UTP accumulation needed for blast transformation and proliferation.
See full target information UCKL1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 13:5945 PubMed36209205

2022

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan Xu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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