AB240160

Anti-UGT1A1 antibody [EPR9592] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal UGT1A1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

GNT1, UGT1, UGT1A1, UDP-glucuronosyltransferase 1A1, Bilirubin-specific UDPGT isozyme 1, UDP-glucuronosyltransferase 1-1, UDP-glucuronosyltransferase 1A isoform 1, hUG-BR1, UDPGT 1-1, UGT1*1, UGT1-01, UGT1.1

2 이미지

Supplier Data

Western blot - Anti-UGT1A1 antibody [EPR9592] - BSA and Azide free (AB240160)

This data was developed using ab170858, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UGT1A1 antibody [EPR9592] (<a href='/ko/products/primary-antibodies/ugt1a1-antibody-epr9592-ab170858'>ab170858</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

LoVo lysate at 10 µg

Predicted band size: 47 kDa,60 kDa

Observed band size: 62 kDa

false

Lab

Western blot - Anti-UGT1A1 antibody [EPR9592] - BSA and Azide free (AB240160)

This data was developed using ab170858, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-UGT1A1 antibody [EPR9592] (<a href='/ko/products/primary-antibodies/ugt1a1-antibody-epr9592-ab170858'>ab170858</a>) at 1/5000 dilution

All lanes:

LoVo (human colorectal adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9592

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab240160 is the carrier-free version of ab170858.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

UGT1A1 also known as UDP-glucuronosyltransferase 1A1 is an enzyme critical for conjugating bilirubin and various hormones with glucuronic acid. It has a molecular mass of approximately 54 kDa. The enzyme primarily localizes in the liver but is also found in other tissues such as the intestine and kidney. UGT1A1's role involves transforming lipophilic molecules into more water-soluble forms facilitating their excretion from the body.

Biological function summary

UGT1A1 contributes to the detoxification processes by mediating the conjugation of bilirubin a byproduct of red blood cell breakdown. This enzyme is part of the UGT1 complex which includes several enzymes sharing a similar expression pattern and function. Through its activity UGT1A1 helps maintain homeostasis and prevents the buildup of potentially toxic substances in the organism.

Pathways

UGT1A1 is a significant component of the glucuronidation pathway. This pathway is essential for phase II metabolism in the liver working alongside other proteins like UGT1A6 and UGT1A9. The glucuronidation pathway transforms hydrophobic compounds into hydrophilic derivatives for easier elimination. Additionally UGT1A1 activity affects hormone metabolism particularly in the pathways involving estrogen and thyroid hormones.

Mutations or polymorphisms in UGT1A1 relate to conditions such as Gilbert’s syndrome and Crigler-Najjar syndrome. These diseases involve impaired bilirubin clearance leading to jaundice. Connection with other proteins like albumin highlights the collaborative role in bilirubin transport and clearance. Studying UGT1A1 and its interactions continues to be critical for understanding and treating these metabolic disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Isoform 1. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed : 12181437, PubMed : 15472229, PubMed : 18004206, PubMed : 18004212, PubMed : 18719240, PubMed : 19830808, PubMed : 23288867, PubMed : 15231852, PubMed : 21422672, PubMed : 38211441). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed : 12181437, PubMed : 18004206, PubMed : 18004212). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol (PubMed : 15472229, PubMed : 18719240, PubMed : 23288867). Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates (PubMed : 17187418, PubMed : 18004206, PubMed : 19830808, PubMed : 24525562). Involved in the glucuronidation of arachidonic acid (AA) and AA-derived eicosanoids including 15-HETE, 20-HETE, PGB1 and F2-isoprostane (8-iso-PGF2alpha) (PubMed : 15231852, PubMed : 38211441). Involved in the glucuronidation of the phytochemical ferulic acid at the phenolic or the carboxylic acid group (PubMed : 21422672). Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (PubMed : 18052087, PubMed : 19545173). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II (PubMed : 18674515). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (PubMed : 12181437, PubMed : 18004212, PubMed : 20610558).. Isoform 2. Lacks UGT glucuronidation activity but acts as a negative regulator of isoform 1.

See full target information UDP-glucuronosyltransferase 1A1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:494-500 PubMed37147750

2023

Electroacupuncture alleviates water avoidance stress-induced irritable bowel syndrome in mice by improving intestinal barrier functions and suppressing the expression of inflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Mengzhu,Zhang Yujie,Song Yafang,Guo Jing,Wang Yuhang,Xin Chen,G U Dongmei,Sun Jianhua,Pei Lixia

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UGT1A1 antibody [EPR9592] - BSA and Azide free

Western blot - Anti-UGT1A1 antibody [EPR9592] - BSA and Azide free (AB240160)

Western blot - Anti-UGT1A1 antibody [EPR9592] - BSA and Azide free (AB240160)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise