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AB317161

Anti-ULK2 antibody [HL2206]

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Rabbit Recombinant Monoclonal ULK2 antibody. Suitable for ICC/IF, WB and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ULK2.

대체 명칭 보기

KIAA0623, ULK2, Serine/threonine-protein kinase ULK2, Unc-51-like kinase 2

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody [HL2206] (AB317161)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody [HL2206] (AB317161)

ab317161 detects ULK2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample : HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : ULK2 stained by ab317161 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-ULK2 antibody [HL2206] (AB317161)
  • WB

Supplier Data

Western blot - Anti-ULK2 antibody [HL2206] (AB317161)

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE and the membrane was blotted with ab317161 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ULK2 antibody [HL2206] (ab317161) at 1/1000 dilution

All lanes:

PC-12 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2206

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ULK2.

Q8IYT8

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보완 제품

Primary Antibodies

AB229537

Anti-ULK1 (phospho S555) antibody

Western blot - Anti-ULK1 (phospho S555) antibody (AB229537)

  • 0
  • 0
Primary Antibodies

AB229327

Anti-LC3B antibody - N-terminal

Lab Essentials

Flow Cytometry - Anti-LC3B antibody - N-terminal (AB229327)

  • 0
  • 0
Primary Antibodies

AB176842

Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade

20ul selling size|RabMAb|Recombinant|Lab Essentials|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (AB176842)

  • 5
  • 15
Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ULK2 also known as UNC-51-like kinase 2 is a serine/threonine-protein kinase positioned within cellular processes. With an approximate molecular mass of 124 kDa ULK2 is expressed in various tissues prominently within the brain heart and skeletal muscle. This protein exhibits kinase activity essential for transferring phosphate groups to specific substrates affecting their function and activity.
Biological function summary

ULK2 functions as an important regulator in cellular autophagy the process that removes unnecessary or damaged cellular components. It acts as part of the ULK complex which includes ATG13 FIP200 and ATG101. This complex initiates the autophagy process by responding to nutrient availability and energy status within the cell effectively managing cellular homeostasis and survival.

Pathways

ULK2 is mainly involved in the autophagy pathway and the mTOR signaling pathway. The mTOR pathway influences cell growth and metabolism in response to nutritional signals hence ULK2 interacts with mTORC1 to relay signals important for appropriate autophagy initiation. Additionally ULK2 shares a functional relationship with AMPK which senses cellular energy levels and modulates autophagy in part through ULK2 activation.

ULK2 is associated with neurodegenerative diseases like Alzheimer's disease and can play a role in the pathology of certain cancers. Aberrant ULK2 activity alters autophagic processes contributing to the accumulation of misfolded proteins and impaired cell survival. The protein interacts with p62/SQSTM1 a known player in neurodegenerative disease pathways underlining its relevance to disease mechanisms.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy : acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation : may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.
See full target information ULK2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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