AB54430

Anti-UNC5B antibody [Guido-1]

Name: Anti-UNC5B antibody [Guido-1]
Brand: Abcam Limited
SKU: AB54430
Rating: 4 (2 reviews)

(2 리뷰들)

(5 제품이 사용된 논문 )

Mouse Monoclonal UNC5B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UNC5B aa 1 to C-terminus.

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (AB54430)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (AB54430)

ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

Guido-1

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human UNC5B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IZJ1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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제품 세부 정보

This product was changed from ascites to tissue culture supernatant on 22^nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from TCS.

보관 버퍼

Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

UNC5B also referred to as Unc-5 homolog B is a member of the UNC5 family of Netrin receptors. This protein acts mechanically as a dependence receptor that modulates axon guidance by mediating repulsion of axons away from the source of its ligand Netrin-1. UNC5B has a molecular mass around 110 kDa. Scientists observe the expression of UNC5B predominantly in endothelial cells and neuronal tissues indicating its role in vascular and nervous system development.

Biological function summary

Scientists understand UNC5B to mediate the anti-angiogenic effects within tissues. It is an essential component of the signaling pathways that govern apoptosis in response to cellular conditions. UNC5B forms part of a larger receptor complex with Netrin-1 which influences cellular directionality and guidance by affecting cytoskeletal dynamics.

Pathways

The UNC5B receptor prominently participates in the Netrin signaling pathway. Within this pathway UNC5B interacts with other proteins such as DCC (deleted in colorectal cancer) that are involved in axonal guidance and neuronal navigation. It also plays a part in the apoptotic signaling pathways which are important for regulating cell survival and programmed cell death.

Researchers associate UNC5B with cancer progression and neurodevelopmental disorders. The regulation of UNC5B is important in the suppression of tumor angiogenesis where its interaction with Netrin-1 can impact cancer cell vascular supply. Additionally misregulation of its pathway might contribute to conditions such as autism spectrum disorders where proteins like DCC interact with it indicating a potential role in neurodevelopmental pathologies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as a netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed : 12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity).

See full target information UNC5B

대체 명칭 보기

P53RDL1, UNC5H2, UNQ1883/PRO4326, UNC5B, Netrin receptor UNC5B, Protein unc-5 homolog 2, Protein unc-5 homolog B, p53-regulated receptor for death and life protein 1, p53RDL1

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 25:2121-2135 PubMed33345442

2020

The intracellular domain of UNC5B facilities proliferation and metastasis of bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yexiang Huang,Zhe Zhang,Miao Miao,Chuize Kong

Translational psychiatry 8:245 PubMed30410030

2018

Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Srikanth,Valentina N Lagomarsino,Richard V Pearse,Meichen Liao,Sulagna Ghosh,Ralda Nehme,Nicholas Seyfried,Kevin Eggan,Tracy L Young-Pearse

The Journal of clinical investigation 126:3006-22 PubMed27400127

2016

Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Khalil Miloudi,François Binet,Ariel Wilson,Agustin Cerani,Malika Oubaha,Catherine Menard,Sullivan Henriques,Gaelle Mawambo,Agnieszka Dejda,Phuong Trang Nguyen,Flavio A Rezende,Steve Bourgault,Timothy E Kennedy,Przemyslaw Sapieha

Nature medicine 20:377-84 PubMed24584118

2014

Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.

Applications

Species

Unspecified reactive species

Bhama Ramkhelawon,Elizabeth J Hennessy,Mickaël Ménager,Tathagat Dutta Ray,Frederick J Sheedy,Susan Hutchison,Amarylis Wanschel,Scott Oldebeken,Michele Geoffrion,Westley Spiro,George Miller,Ruth McPherson,Katey J Rayner,Kathryn J Moore

PloS one 7:e41085 PubMed22848430

2012

The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury.

Applications

Flow Cyt

Species

Mouse

Klemens König,David Köhler,Tiago Granja,Carla Jennewein,Nguyen Tran,Valbona Mirakaj,Friedemann Kröhnert,Peter Rosenberger

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UNC5B antibody [Guido-1]

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (AB54430)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise