AB157195

Anti-UPB1 antibody [EPR9132]

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal UPB1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UPB1 antibody [EPR9132] (AB157195)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UPB1 antibody [EPR9132] (AB157195)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UPB1 with ab157195 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UPB1 antibody [EPR9132] (AB157195)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UPB1 with ab157195 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-UPB1 antibody [EPR9132] (AB157195)

Detection of UPB1 by Western Blot of Immunprecipitate. Human fetal liver lysate immunoprecipitated using ab157195 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-UPB1 antibody [EPR9132] (ab157195)

Predicted band size: 43 kDa

false

Unknown

Western blot - Anti-UPB1 antibody [EPR9132] (AB157195)

All lanes:

Western blot - Anti-UPB1 antibody [EPR9132] (ab157195) at 1/1000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9132

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

UPB1 also known as Ureidopropionase beta is an enzyme encoded by the UPB1 gene. It is approximately 37 kDa in mass. UPB1 conducts the hydrolysis of N-carbamoyl-beta-alanine and N-carbamoyl-beta-aminoisobutyric acid to provide beta-alanine beta-aminoisobutyric acid ammonia and carbon dioxide. It is commonly expressed in liver and kidney tissues.

Biological function summary

UPB1 plays an important role in the catabolic pathway of pyrimidine metabolism. This enzyme participates in the breakdown of uracil which is part of nucleotide catabolism. UPB1 operates independently rather than as a part of a protein complex. It ensures detoxification and recycling of nitrogenous compounds derived from pyrimidine breakdown.

Pathways

UPB1 is an integral component of the pyrimidine metabolic pathway. It provides a link between the pyrimidine degradation pathway and general nitrogen metabolism by supplying beta-alanine. This function complements the enzymatic roles of dihydropyrimidine dehydrogenase (DPYD) and dihydropyrimidinase (DPYS) which are involved in earlier steps in the same degradation pathway.

Disturbances associated with UPB1 can lead to a condition called N-carbamoyl-beta-amino aciduria. This is a metabolic disorder characterized by excessive accumulation of N-carbamoyl-beta-amino acids in urine. The interaction with proteins such as DPYS and DPYD may exacerbate or modify the severity of this metabolic condition as they are upstream components in the pyrimidine degradation pathway.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes a late step in pyrimidine degradation (PubMed : 22525402, PubMed : 24526388). Converts N-carbamoyl-beta-alanine (3-ureidopropanoate) into beta-alanine, ammonia and carbon dioxide (PubMed : 10415095, PubMed : 10542323, PubMed : 11508704, PubMed : 22525402, PubMed : 24526388, PubMed : 29976570). Likewise, converts N-carbamoyl-beta-aminoisobutyrate (3-ureidoisobutyrate) into beta-aminoisobutyrate, ammonia and carbon dioxide (Probable).

See full target information UPB1

대체 명칭 보기

BUP1, UPB1, Beta-ureidopropionase, BUP-1, Beta-alanine synthase, N-carbamoyl-beta-alanine amidohydrolase

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 13:993187 PubMed36119068

2022

A robust CD8 T cell-related classifier for predicting the prognosis and efficacy of immunotherapy in stage III lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinteng Feng,Longwen Xu,Shirong Zhang,Luying Geng,Tian Zhang,Yang Yu,Rui Yuan,Yusheng He,Zhuhui Nan,Min Lin,Hui Guo

Scientific reports 7:5517 PubMed28717245

2017

Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma.

Applications

WB, IHC

Species

Unspecified reactive species, Unspecified reactive species

Binghua Li,Wendu Feng,Ouyang Luo,Tiancheng Xu,Yajuan Cao,Hongyan Wu,Decai Yu,Yitao Ding

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UPB1 antibody [EPR9132]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UPB1 antibody [EPR9132] (AB157195)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UPB1 antibody [EPR9132] (AB157195)

Immunoprecipitation - Anti-UPB1 antibody [EPR9132] (AB157195)

Western blot - Anti-UPB1 antibody [EPR9132] (AB157195)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise