AB263454

Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081]

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(2 제품이 사용된 논문 )

Mouse Monoclonal Uroplakin Ib/UPIb antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UPK1B aa 100-250.

대체 명칭 보기

TSPAN20, UPK1B, Uroplakin-1b, UP1b, Tetraspanin-20, Uroplakin Ib, Tspan-20, UPIb

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263454 at 2 μg/ml in immunohistochemistry.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263454 at 2 μg/ml in immunohistochemistry.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263454 at 2 μg/ml in immunohistochemistry.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

UPK1B/3081

Isotype

IgG2b

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UPK1B aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

O75841

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with the primary antibody for 30 mins at RT.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from bioreactor concentrate.

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Uroplakin Ib (UPIb) also known as UPK1B is a type I integral membrane protein belonging to the uroplakin family. UPIb has a molecular mass of around 25 kDa. It is expressed in the apical surface of urothelial cells which line the urinary bladder and other parts of the urinary tract. The protein plays a structural role contributing to the formation of the asymmetrical unit membrane (AUM) an important component of the urothelial barrier.

Biological function summary

UPIb functions as part of a larger uroplakin complex essential for maintaining the urothelial surface structure. This protein works in concert with Uroplakin Ia Uroplakin II and Uroplakin III to form UPIa/UPIb heterodimers and facilitate protective plaque formation on the urothelial surface. The uroplakin complex ensures impermeability and mechanical stability in the urothelium critically protecting the underlying tissues from urine's toxic and osmotic effects.

Pathways

UPIb plays an essential role in the processes of cell differentiation and urothelial development. It interacts with transmembrane proteins within the uroplakin complex supporting the endocytosis and recycling pathways in the bladder's epithelial cells. The protein influences pathways pertinent to epithelial cell adhesion and communication working closely with uroplakin-associated proteins such as Uroplakin II and Uroplakin IIIa.

UPIb is linked to bladder-related conditions due to its involvement in maintaining the urothelial barrier's integrity. Defects or misregulation in UPIb expression have associations with bladder cancer and interstitial cystitis. In bladder cancer abnormal uroplakin expression patterns including UPIb may indicate changes in the differentiation state of cancerous cells. In interstitial cystitis impaired urothelial barrier functions may link to UPIb's disrupted interactions with proteins such as Uroplakin IIIa.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).

See full target information UPK1B

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 24: PubMed37958809

2023

mRNA and Protein Expression in Human Fetal Membrane Cells: Potential Biomarkers for Preterm Prelabor Rupture of the Fetal Membranes?

Applications

Unspecified application

Species

Unspecified reactive species

Emmeli Mikkelsen,Berthold Huppertz,Ripudaman Singh,Katarina Ravn,Lotte Hatt,Mogens Kruhøffer,Rheanna Urrabaz-Garza,Niels Uldbjerg,Ramkumar Menon,Torben Steiniche

Cancer research 69:8111-9 PubMed19808967

2009

Runt-related transcription factor RUNX3 is a target of MDM2-mediated ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Zi Chi,Jiyeon Kim,Yong-Hee Lee,Jung-Won Lee,Kyeong-Sook Lee,Heejun Wee,Wun-Jae Kim,Woo-Yoon Park,Byung-Chul Oh,Gary S Stein,Yoshiaki Ito,Andre J van Wijnen,Suk-Chul Bae

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [UPK1B/3081] (AB263454)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise