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AB252955

Anti-UROS antibody

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Rabbit Polyclonal UROS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UROS aa 100-250.

대체 명칭 보기

Uroporphyrinogen-III synthase, UROIIIS, UROS, Hydroxymethylbilane hydrolyase [cyclizing], Uroporphyrinogen-III cosynthase

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UROS antibody (AB252955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UROS antibody (AB252955)

Paraffin-embedded human colon tissue stained for UROS using ab252955 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-UROS antibody (AB252955)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UROS antibody (AB252955)

PFA-fixed, Triton X-100 permeabilized U-2OS (human brain glioma cell line) cells stained for UROS (green) using ab252955 at 4 μg/ml in ICC/IF.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UROS aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P10746

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보완 제품

Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

  • 4
  • 3
Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

  • 0
  • 0
Proteins & Peptides

AB107136

Recombinant Human UROS protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human UROS protein (His tag N-Terminus) (AB107136)

  • 0
  • 0
Sample Prep & Detection Kits

AB201807

APC Conjugation Kit - Lightning-Link®

Fluorescence Microscopy - APC Conjugation Kit - Lightning-Link® (AB201807)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Uroporphyrinogen III synthase commonly known as UROS is an enzyme involved in heme biosynthesis. It catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III facilitating a ring closure and isomerization. UROS has a molecular mass of approximately 29 kDa. It is predominantly expressed in the liver bone marrow and erythroid tissues where heme production is essential. The enzyme is vital for maintaining proper levels of porphyrins in cells and ensuring the biosynthesis of heme an important component of hemoglobin.
Biological function summary

Uroporphyrinogen III synthase plays a role in heme production converting linear tetrapyrroles into the cyclic form needed for biosynthesis. This enzyme does not function as part of a larger complex but rather works independently to catalyze its specific reaction in porphyrin metabolism. Proper function of UROS is necessary to sustain the production of heme which is critical not only for oxygen transport in the blood but also for other heme-dependent processes in various tissues.

Pathways

UROS is an essential component of the heme biosynthesis pathway. In this pathway it acts between the steps that involve hydroxymethylbilane synthase (HMBS) and uroporphyrinogen III decarboxylase (UROD). This metabolic pathway is important for the production of heme which is a core component of hemoglobin. HMBS synthesizes the precursor transformed by UROS while UROD further modifies the product of UROS to continue the biosynthesis process emphasizing the enzyme's importance in maintaining the heme biosynthesis pathway's integrity.

Mutations or deficiencies in UROS are linked to a rare inherited condition called congenital erythropoietic porphyria (CEP) also known as Günther's disease. This disorder results from the accumulation of porphyrins owing to impaired heme biosynthesis. Abnormal function in UROS leads to a buildup of its substrates causing severe skin photosensitivity and hemolytic anemia among other symptoms. The functional relationship between UROS and HMBS or UROD in the heme biosynthesis pathway illustrates the enzyme's significant role in preventing the development of such heme-related disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins (PubMed : 11689424, PubMed : 18004775). Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme) (PubMed : 11689424, PubMed : 18004775).
See full target information UROS
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:6876 PubMed40715139

2025

The mechanotransducer Piezo1 coordinates metabolism and inflammation to promote skin growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Xue,Elizabeth Winnicki,Zhaoxu Zhang,Ines Lopez,Saifeng Wang,Charles Kirby,Sam S Lee,Ang Li,Chaewon Lee,Hana Minsky,Kaitlin Williams,Kevin Yueh-Hsun Yang,Ling He,Sashank K Reddy,Luis A Garza
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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