AB4850

Anti-USP15 antibody

Name: Anti-USP15 antibody
Brand: Abcam Limited
SKU: AB4850
Rating: 3 (1 reviews)

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(2 제품이 사용된 논문 )

Goat Polyclonal USP15 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human USP15 aa 950 to C-terminus.

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (AB4850)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (AB4850)

Immunohistochemical analysis of paraffin embedded Human Liver labeling USP15 with ab4850 at 2.5-3.8μg/ml. The image shows patterned intracellular staining in hepatocytes.

Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human USP15 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4E8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-3.8 µg/mL", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with citrate buffer (pH6).</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Gene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; deubiquitination.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

USP15 also known as Ubiquitin Specific Peptidase 15 is a deubiquitinating enzyme that removes ubiquitin from target proteins a process integral to protein degradation and regulation. It has a molecular mass of approximately 107 kDa. USP15 regulates protein turnover and stability through its enzymatic activity and is predominantly cytoplasmic expressed in various tissues including the brain heart and liver. Its expression pattern suggests widespread functionality across different physiological contexts.

Biological function summary

USP15 is involved in numerous cellular processes through its role in protein homeostasis. It associates with several protein complexes to regulate signaling pathways and cellular responses. USP15 plays a central role in the regulation of TGF-β signaling by deubiquitinating and stabilizing SMAD proteins that are important for signal transduction. In addition to TGF-β modulation USP15 also participates in endocytic trafficking by associating with the ESCRT complex impacting receptor degradation and cell signaling.

Pathways

The function of USP15 is critical in integrating cellular signaling networks such as the TGF-β and NF-kB pathways. Within the TGF-β pathway USP15 deubiquitinates receptor-regulated SMADs maintaining their stability and enhancing signal transduction. In the NF-kB pathway USP15 influences the activation by regulating the degradation of IκBα an inhibitor of NF-kB which indicates its role in immune response and inflammation. Through these pathways USP15 interacts with proteins like SMAD2/3 in TGF-β signaling and IκBα in NF-kB regulation.

USP15 has associations with cancer and neurodegenerative diseases. In cancer altered USP15 activity leads to dysregulation of TGF-β signaling influencing tumor progression and metastasis. The enzyme interacts with proteins such as MDM2 in neurodegenerative disorders affecting p53 stability and neuronal cell survival. This suggests a role in processes like apoptosis and DNA repair critical in the onset and progression of neurodegenerative conditions. But current research continues aiming for better understanding of USP15's connections with these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004, PubMed : 21947082, PubMed : 22344298, PubMed : 24852371). Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear : according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed : 21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed : 22344298). Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 33093067). May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed : 24526689). Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN) : hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy (PubMed : 24852371). Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association : regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004). Involved in endosome organization by mediating deubiquitination of SQSTM1 : ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery (PubMed : 27368102). Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9 (PubMed : 33093067).. (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.

See full target information USP15

대체 명칭 보기

KIAA0529, USP15, Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in aging neuroscience 17:1549770 PubMed40365352

2025

Dysregulated calcium signaling in the aged macaque entorhinal cortex associated with tau hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Shveta Bathla,Dibyadeep Datta,Dinara Bolat,Elizabeth Woo,Alvaro Duque,Jon I Arellano,Amy F T Arnsten,Angus C Nairn

Frontiers in cell and developmental biology 8:529 PubMed32671073

2020

USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yixuan Zhao,Xin Huang,Zewei Zhang,Yifan Zhang,Guoyou Zhang,Tao Zan,Qingfeng Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP15 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (AB4850)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise