Anti-USP30 antibody [CL4438]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP30 antibody [CL4438] (AB219969)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling USP30 with ab219969 at 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP30 antibody [CL4438] (AB219969)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling USP30 with ab219969 at 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP30 antibody [CL4438] (AB219969)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling USP30 with ab219969 at 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP30 antibody [CL4438] (AB219969)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling USP30 with ab219969 at 1/200 dilution.

• WB

Lab

Western blot - Anti-USP30 antibody [CL4438] (AB219969)

Western blot : Mouse Monoclonal [CL4438] to USP30 ab219969 staining at 1/1000 dilution, shown in green; Rabbit anti GAPDH (ab181602) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 59 kDa in Wild-type A549 ab288558 cell lysates with no signal observed at this size in USP30 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-USP30 antibody [CL4438] (ab219969) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human USP30 Knockout A549 cell line (<a href='/ko/products/cell-lines/human-usp30-knockout-a549-cell-line-ab324260'>ab324260</a>) at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

false

• WB

Lab

Western blot - Anti-USP30 antibody [CL4438] (AB219969)

Western blot : Mouse Monoclonal[CL4438] to USP30 ab219969 staining at 1/1000 dilution, shown in green; Rabbit anti GAPDH (ab181602) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 59 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in USP30 knockout MCF7 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-USP30 antibody [CL4438] (ab219969) at 1/1000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human USP30 knockout MCF7 cell line (<a href='/ko/products/cell-lines/human-usp30-knockout-mcf7-cell-line-ab324541'>ab324541</a>) at 20 µg

Lane 3:

Wild-type A549 ab288558 at 20 µg

Lane 4:

USP30 knockout A549 at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

false