AB236987

Anti-USP4 antibody

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(6 제품이 사용된 논문 )

Rabbit Polyclonal USP4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP4 aa 600-850.

대체 명칭 보기

UNP, UNPH, USP4, Ubiquitin carboxyl-terminal hydrolase 4, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP4 antibody (AB236987)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP4 antibody (AB236987)

Paraffin-embedded human colon cancer tissue stained for USP4 using ab236987 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4° overnight. The primary is detected by a biotinylated secondary and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody (AB236987)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody (AB236987)

U-251 MG (Human brain glioma cell line) cells stained for USP4 (green) using ab236987 at 1/166 dilution in ICC/IF, followed by Alexa Fluor^®488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubate with primary antibody at 4°C overnight.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP4 antibody (AB236987)

Paraffin-embedded human kidney tissue stained for USP4 using ab236987 at 1/500 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human USP4 aa 600-850. The exact immunogen used to generate this antibody is proprietary information.

Q13107

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

USP4 also known as Ubiquitin Specific Peptidase 4 is a deubiquitinating enzyme with an approximate mass of 108 kDa. It functions by removing ubiquitin from protein substrates impacting their stability and functionality. USP4 is expressed in various tissues including heart lung skeletal muscle liver and brain. Its enzymatic action is important for regulating protein turnover and preventing the degradation of proteins that require fine control.

Biological function summary

USP4 contributes to multiple cellular processes by maintaining protein homeostasis. It participates in regulation of the endoplasmic reticulum-associated degradation (ERAD) pathway and influences processes such as cellular transport and signal transduction. USP4 often functions within protein complexes that control these pathways including interactions with the spliceosome machinery which impact RNA processing and gene expression.

Pathways

USP4 plays a significant role in the TGF-beta signaling pathway and the WNT signaling pathway. It interacts with proteins such as SMAD2 SMAD3 and beta-catenin which are integral to these pathways for regulating transcriptional activity and cellular responses. Through the regulation of signaling components USP4 modulates key developmental and cellular growth processes.

USP4 has been linked to cancer and neurodegenerative diseases. Overexpression of USP4 is observed in certain cancers such as breast cancer where it affects the stability of proteins like TGF-beta receptor influencing tumor progression. In neurodegenerative diseases USP4's interaction with proteins like tau can contribute to the pathology as defects in protein clearance mechanisms play a role in disease development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins (PubMed : 16316627, PubMed : 16339847, PubMed : 16472766, PubMed : 20595234, PubMed : 22347420, PubMed : 25404403, PubMed : 28604766, PubMed : 30514904). Deubiquitinates PDPK1 (PubMed : 22347420). Deubiquitinates TRIM21 (PubMed : 16316627). Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface (PubMed : 16339847). Deubiquitinates HAS2 (PubMed : 28604766). Deubiquitinates MAVs leading to maintain MAVS protein stability, resulting in increased production of type I interferons (IFN-I) and enhanced antiviral innate immune responses against viral infections (PubMed : 39589880). Deubiquitinates RHEB in response to EGF signaling, promoting mTORC1 signaling (PubMed : 30514904). May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3 (PubMed : 20595234). This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP (PubMed : 20595234). May also play a role in the regulation of quality control in the ER (PubMed : 16339847).

See full target information USP4

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:11096 PubMed40169699

2025

USP4 promotes proliferation and metastasis in human lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yamin Wei,Shanwang Wei,Zhongteng Lei,Yan Zhang,Jinxiao Wu,Jinli Huang,Lijuan Fu,Zhimeng Li,Guiying Huang,Yuanna Liang,Jinhua Zheng

Journal of orthopaedic surgery and research 20:220 PubMed40022160

2025

A novel mechanism in regulating drug sensitivity, growth, and apoptosis of bortezomib-resistant multiple myeloma cells: the USP4/KLF2/HMGA2 cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Liu,Xiaoliang Li,Yin He,Fengqun Xie,Yanrong Zhang,Mufang Huang,Yinjingwen Wang,Weijian Zhu

Cell death & disease 16:81 PubMed39922805

2025

USP4-mediated CENPF deubiquitylation regulated tumor metastasis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongdong Xie,Hanbin Lin,Yuecheng Wu,Yanan Yu,Xintong Liu,Yating Zheng,Xiaojie Wang,Jiashu Wu,Meifang Xu,Yuting Han,Qiongying Zhang,Yu Deng,Lin Lin,Yan Linzhu,Li Qingyun,Xinjian Lin,Ying Huang,Pan Chi

iScience 27:109675 PubMed38706837

2024

extract attenuates corticotropin-releasing hormone-induced cellular senescence in human hair follicle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sunhyoung Lee,So Young Kim,Seunghee Lee,Sunhyae Jang,Sungjoo Tommy Hwang,Youngji Kwon,Jaehwan Choi,Ohsang Kwon

Molecular therapy. Nucleic acids 23:200-214 PubMed33376627

2020

SOX2-Upregulated microRNA-30e Promotes the Progression of Esophageal Cancer via Regulation of the USP4/SMAD4/CK2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Xin Fan,Yukai Ren,Kai Wu,Xiangyu Tian,Fengbiao Wen,Donglei Liu,Yuxia Fan,Song Zhao

Medical science monitor : international medical jo 26:e920736 PubMed32308208

2020

The Effects of the Transforming Growth Factor-β1 (TGF-β1) Signaling Pathway on Cell Proliferation and Cell Migration are Mediated by Ubiquitin Specific Protease 4 (USP4) in Hypertrophic Scar Tissue and Primary Fibroblast Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Huang,Yuting Wang,Xueming Wang,Lixin Lin,Peng Wang,Junjun Sun,Lei Jiang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP4 antibody (AB236987)

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody (AB236987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP4 antibody (AB236987)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise