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AB121254

Anti-USP42 antibody

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Rabbit Polyclonal USP42 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human USP42 aa 450-600.

대체 명칭 보기

Ubiquitin carboxyl-terminal hydrolase 42, Deubiquitinating enzyme 42, Ubiquitin thioesterase 42, Ubiquitin-specific-processing protease 42, USP42

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-USP42 antibody (AB121254)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USP42 antibody (AB121254)

Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121254 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP42 antibody (AB121254)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP42 antibody (AB121254)

Immunohistochemical staining of parffin embedded Human kidney, using 1/60 ab121254, shows strong nuclear cytoplasmic positivity in cells in tubules and cells in glomeruli.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human USP42 aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

Q9H9J4

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보완 제품

Primary Antibodies

AB190184

Anti-USP37 antibody

Western blot - Anti-USP37 antibody (AB190184)

  • 0
  • 0
Primary Antibodies

AB124814

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814)

  • 4
  • 1
Primary Antibodies

AB254939

Anti-USP35 antibody

Immunocytochemistry/ Immunofluorescence - Anti-USP35 antibody (AB254939)

  • 0
  • 0
Primary Antibodies

AB109315

Anti-Cdc6 antibody [EPR714(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (AB109315)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p>Use buffer at pH 6 for antigen retrieval.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

USP42 also known as Ubiquitin-specific peptidase 42 is part of the ubiquitin-proteasome system. USP42 specifically removes ubiquitin molecules from specific protein substrates. This action involves deubiquitination a process that regulates protein degradation. The molecular weight of USP42 is approximately 150 kDa. In terms of expression USP42 is found in various tissues including the liver brain and kidneys indicating it has a broad role across different systems of the body.
Biological function summary

The function of USP42 is important in gene regulation and cell cycle control. By controlling protein stability it influences transcriptional activity and cellular responses to stress. USP42 associates with the p53 protein stabilizing it and enhancing its tumor suppressor function. Additionally it forms part of larger protein complexes that coordinate DNA damage responses. Through these interactions USP42 supports cellular health by ensuring proper repair and replication of DNA.

Pathways

In these pathways USP42 plays a part in modulating ubiquitin ligases and deubiquitinases activities maintaining protein homeostasis. The protein acts in concert with others like Mdm2 and p53 important for cellular stress responses and apoptosis. These pathways highlight the impact of USP42 in balancing cell survival and programmed cell death mechanisms.

Altered USP42 expression or function has links to tumorigenesis due to its interaction with proteins such as p53. Dysregulation in this pathway may contribute to cancer proliferation. In neurodegenerative conditions abnormal protein aggregation occurs if ubiquitin-proteasome function is impaired connecting to USP42's role in maintaining protein quality control. The protein's dysfunction can therefore influence disease progression by failing to regulate protein turnover properly.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Deubiquitinating enzyme which may play an important role during spermatogenesis.
See full target information USP42
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell stem cell 22:851-864.e5 PubMed29804889

2018

An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Du,Mehdi Pirouz,Jiho Choi,Aaron J Huebner,Kendell Clement,Alexander Meissner,Konrad Hochedlinger,Richard I Gregory
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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