AB237765

Anti-USP48 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal USP48 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP48 aa 350-550.

대체 명칭 보기

USP31, USP48, Ubiquitin carboxyl-terminal hydrolase 48, Deubiquitinating enzyme 48, Ubiquitin thioesterase 48, Ubiquitin-specific peptidase 48, Ubiquitin-specific protease 48, Ubiquitin-specific-processing protease 48

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP48 antibody (AB237765)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP48 antibody (AB237765)

Paraffin-embedded human small intestine tissue stained for USP48 with ab237765 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP48 antibody (AB237765)

Paraffin-embedded human pancreatic cancer tissue stained for USP48 with ab237765 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-USP48 antibody (AB237765)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP48 antibody (AB237765)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for USP48 (green) using ab237765 at 1/66 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human USP48 aa 350-550. The exact immunogen used to generate this antibody is proprietary information.

Q86UV5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity greater than 95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

USP48 also known as Ubiquitin Specific Peptidase 48 acts as a deubiquitinating enzyme. It specifically removes ubiquitin from target proteins influencing protein stability and function. USP48 has a molecular mass of approximately 130 kDa. This enzyme expresses in various tissues with significant levels observed in the brain and immune cells. Researchers recognize its involvement in protein homeostasis through regulation of protein degradation pathways.

Biological function summary

Protein deubiquitination by USP48 affects cellular processes such as DNA repair transcription regulation and signal transduction. USP48 does not function as part of a stable complex but it associates transiently with substrates and other molecular partners during its activity. It has a role in maintaining genomic stability by modulating transcription factors and repair proteins through deubiquitination.

Pathways

One can find USP48 participating in the NF-kB signaling pathway. It stabilizes RelA an important NF-kB subunit by removing ubiquitin which influences the transcription of genes involved in immune response and inflammation. USP48 also interacts with proteins such as OTUB1 in these pathways contributing to the balance of protein ubiquitination and deubiquitination within the cell.

Research connections exist between USP48 and several cancers including glioblastoma due to its regulation of pathways that control cell survival and proliferation. USP48's interactions with NF-kB impact glioblastoma progression by altering the expression of genes that promote cell growth. Defective USP48 activity also associates with neurodegenerative disorders where its modulation of protein stability could contribute to neuron degeneration through inadequate DNA repair mechanisms.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Deubiquitinase that recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of polyubiquitin precursors as well as that of ubiquitinated proteins (PubMed : 16214042, PubMed : 34059922). Plays a role in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. Plays an important role in cell cycle progression by deubiquitinating Aurora B/AURKB and thereby extending its stability (PubMed : 34445214). In the context of H.pylori infection, stabilizes nuclear RELA through deubiquitination, thereby promoting the transcriptional activity of RELA to prolong TNFAIP3 de novo synthesis. Consequently, TNFAIP3 suppresses caspase activity and apoptotic cell death (PubMed : 35913642). Also functions in the modulation of the ciliary and synaptic transport as well as cytoskeleton organization, which are key for photoreceptor function and homeostasis. To achieve this, stabilizes the levels of the retinal degeneration-associated proteins ARL3 and UNC119 using distinct mechanisms (PubMed : 36293380). Plays a positive role in pyroptosis by stabilizing gasdermin E/GSDME through removal of its 'Lys-48'-linked ubiquitination (PubMed : 36607699).

See full target information USP48

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC complementary medicine and therapies 24:42 PubMed38245760

2024

Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in HO-induced granulosa cells apoptosis via USP48 protein regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Chen,Chuhua Lin,Xiurong Huang,Wei Bian

Aging cell 22:e14009 PubMed37960952

2023

3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zer Vue,Edgar Garza-Lopez,Kit Neikirk,Prasanna Katti,Larry Vang,Heather Beasley,Jianqiang Shao,Andrea G Marshall,Amber Crabtree,Alexandria C Murphy,Brenita C Jenkins,Praveena Prasad,Chantell Evans,Brittany Taylor,Margaret Mungai,Mason Killion,Dominique Stephens,Trace A Christensen,Jacob Lam,Benjamin Rodriguez,Mark A Phillips,Nastaran Daneshgar,Ho-Jin Koh,Alice Koh,Jamaine Davis,Nina Devine,Mohammad Saleem,Estevão Scudese,Kenneth Ryan Arnold,Valeria Vanessa Chavarin,Ryan Daniel Robinson,Moumita Chakraborty,Jennifer A Gaddy,Mariya T Sweetwyne,Genesis Wilson,Elma Zaganjor,James Kezos,Cristiana Dondi,Anilkumar K Reddy,Brian Glancy,Annet Kirabo,Anita M Quintana,Dao-Fu Dai,Karen Ocorr,Sandra A Murray,Steven M Damo,Vernat Exil,Blake Riggs,Bret C Mobley,Jose A Gomez,Melanie R McReynolds,Antentor Hinton

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP48 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP48 antibody (AB237765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP48 antibody (AB237765)

Immunocytochemistry/ Immunofluorescence - Anti-USP48 antibody (AB237765)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise