AB53418

Anti-V5 tag antibody [E10]

Name: Anti-V5 tag antibody [E10]
Brand: Abcam Limited
SKU: AB53418
Rating: 4 (6 reviews)

Lab Essentials
각 태그의 뜻

(6 리뷰들)

(6 제품이 사용된 논문 )

Mouse Monoclonal V5 tag antibody. Suitable for ELISA, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Tag samples. Cited in 6 publications.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (AB53418)

ICC/IF

AbReview39525****

Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (AB53418)

ab53418 staining V5 tag in Mouse B16F1 melanoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Tween 20 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 1% BSA + 0.5% Tween 20 + 1% Donkey serum) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

Melonma cells expressing GFP are also expressing GFP (AF55) due to transfected cell line with GFP-V5.

This image is courtesy of an Abreview submitted by Javier Rodriguez

Unknown

Western blot - Anti-V5 tag antibody [E10] (AB53418)

All lanes:

Western blot - Anti-V5 tag antibody [E10] (ab53418) at 1/1000 dilution

Lane 1:

293 cellsuntransfected (control) at 30 µg

Lane 2:

293 cellstransfectedwith lacZ-V5-tagged at 30 µg

Observed band size: 130 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

E10

Isotype

IgG1

Carrier free

Applications

WB, IHC-P, IHC-Fr, ELISA, ICC/IF

applications

Specificity

ab53418 detects (GKPIPNPLLGLDST) V5 tag fusion protein.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/5000 - 1/10000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/2000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/500 - 1/2000", "IHCFr-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

ab53418 was affinity purified from culture supernatant of hybridoma cells grown in a bioreactor.

보관 버퍼

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.

Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information V5 tag

대체 명칭 보기

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature 570:533-537 PubMed31217585

2019

A universal biomolecular integral feedback controller for robust perfect adaptation.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie K Aoki,Gabriele Lillacci,Ankit Gupta,Armin Baumschlager,David Schweingruber,Mustafa Khammash

Nucleic acids research 47:5381-5394 PubMed30931476

2019

Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Mora Gallardo,Ainhoa Sánchez de Diego,Julio Gutiérrez Hernández,Amaia Talavera-Gutiérrez,Thierry Fischer,Carlos Martínez-A,Karel H M van Wely

Cell 167:219-232.e14 PubMed27662090

2016

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.

Applications

Species

Unspecified reactive species

Angelo Amabile,Alessandro Migliara,Paola Capasso,Mauro Biffi,Davide Cittaro,Luigi Naldini,Angelo Lombardo

Insect biochemistry and molecular biology 54:80-8 PubMed25240618

2014

Sumoylation modulates 20-hydroxyecdysone signaling by maintaining USP protein levels in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawan Wang,Sheng Wang,Sheng Li

Archives of toxicology 89:2027-38 PubMed25224401

2014

Novel role of hnRNP-A2/B1 in modulating aryl hydrocarbon receptor ligand sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

See-Wun Cho,Ken-ichi Suzuki,Yoshiaki Miura,Tatsuhiko Miyazaki,Masato Nose,Hisato Iwata,Eun-Young Kim

Journal of virology 84:10681-9 PubMed20702639

2010

The African swine fever virus DP71L protein recruits the protein phosphatase 1 catalytic subunit to dephosphorylate eIF2alpha and inhibits CHOP induction but is dispensable for these activities during virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Fuquan Zhang,Alice Moon,Kay Childs,Stephen Goodbourn,Linda K Dixon

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-V5 tag antibody [E10]

Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (AB53418)

Western blot - Anti-V5 tag antibody [E10] (AB53418)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise