Anti-V5 tag antibody [SV5-Pk1]

• WB

AbReview55266****

Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)

Cells were transfected with a V5-tagged HSPB8 (MW : ~25kD) plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.

All lanes:

Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution

Lanes 1 - 2:

Transfected NIH/3T3 cell lysate

Lane 3:

Not transfected NIH/3T3 cell lysate

Secondary

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HRP-conjugated rabbit polyclonal Anti-mouse

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Exposure time: 1s

• WB

PubMed

Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)

Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.

(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.

All lanes:

Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)

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Flores et al PLoS One. 2016 Oct 24;11(10):e0165084. doi: 10.1371/journal.pone.0165084. eCollection 2016. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

CiteAb

Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)

V5 tag western blotting using Anti-V5 tag antibody [SV5-Pk1] ab27671. Publication image and figure legend from Moura, D., Campillo-Marcos, I., et al., 2018 ,Cell Mol Life Sci Pubmed 29340707.

VRK1 contributes to the stability of AURKB. a 293T cells were transfected first with SiControl or SiVRK1-02 and 24 h later transfected using PEI with AURKB (pcDNA3.1/nV5-DEST-AURKB) in combination with V5 and with active HA-ubiquitin (pSSK-HA--Ubiquitin). The immunoprecipitated proteins were determined in immunoblots. b The 293T cells were transfected with plasmids expressing HA-VRK1 (pCEFL-HA-VRK1) and V5-tagged AURKB. 72 h after transfection and overexpression, the 293T cells were treated with 50 μg/mL of cycloheximide and aliquots were taken at different time points during 24 h for protein level determinations. The two experiments were performed three times and the error bars represent the standard deviations. The dashed line represents the 50% level

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