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AB326355

Anti-VAChT antibody [S6]

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Mouse Recombinant Monoclonal VACHT antibody. Suitable for ICC/IF, WB and reacts with Human, Rat samples.
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Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody [S6] (AB326355)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody [S6] (AB326355)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Neuroblastoma cell line (SK-N-BE). Species : Human. Fixation : 4% Formaldehyde for 15 min at RT. Primary Antibody : ab326355 at 1 : 100 for 60 min at RT. Secondary Antibody : Goat Anti-Mouse ATTO 488 at 1 : 200 for 60 min at RT. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1 : 1000, 1 : 5000 for 60 min at RT, 5 min at RT. Localization : Membrane. Magnification : 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab326355. (D) Composite.

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody [S6] (AB326355)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody [S6] (AB326355)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Neuroblastoma cells (SH-SY5Y). Species : Human. Fixation : 4% PFA for 15 min. Primary Antibody : ab326355 at 1 : 200 for overnight at 4°C with slow rocking. Secondary Antibody : AlexaFluor 488 at 1 : 1000 for 1 hour at RT. Counterstain : Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1 : 800, 1.6mM for 20 min at RT.

Western blot - Anti-VAChT antibody [S6] (AB326355)
  • WB

Supplier Data

Western blot - Anti-VAChT antibody [S6] (AB326355)

Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein. Primary Antibody : ab326355 at 1 : 1000.

All lanes:

Western blot - Anti-VAChT antibody [S6] (ab326355) at 1/1000 dilution

All lanes:

Rat brain membrane lysate

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

S6

Isotype

IgG1

Carrier free

No

Reacts with

Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB86501

Anti-Islet 1 antibody [1B1] - Neural Stem Cell Marker

BOND RX™ Validated|KO Validated

Western blot - Anti-Islet 1 antibody [1B1] - Neural Stem Cell Marker (AB86501)

  • 5
  • 2
Primary Antibodies

AB109535

Anti-TDP43 antibody [EPR5810]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-TDP43 antibody [EPR5810] (AB109535)

  • 5
  • 1
Primary Antibodies

AB195887

Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

  • 4
  • 1
Primary Antibodies

AB181023

Anti-Choline Acetyltransferase antibody [EPR13024(B)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] (AB181023)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis (By similarity) (PubMed : 20225888, PubMed : 8910293). Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern (By similarity). Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation (By similarity). Can transport serotonin.
See full target information SLC18A3

대체 명칭 보기

VACHT, SLC18A3, Vesicular acetylcholine transporter, VAChT, Solute carrier family 18 member 3, Vacht, Vat, Vesicular acetylcholine transporter, VAChT, rVAT, Solute carrier family 18 member 3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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