AB60172

Anti-VANGL2 antibody

Name: Anti-VANGL2 antibody
Brand: Abcam Limited
SKU: AB60172
Rating: 3 (2 reviews)

(2 리뷰들)

(2 제품이 사용된 논문 )

Goat Polyclonal VANGL2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human VANGL2 aa 400-450.

1 이미지

Unknown

Western blot - Anti-VANGL2 antibody (AB60172)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-VANGL2 antibody (ab60172) at 0.3 µg/mL

All lanes:

Mouse Brain lysate (35µg protein in RIPA buffer)

Predicted band size: 60 kDa

Observed band size: 55 kDa

false

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human VANGL2 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q9ULK5

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

VANGL2 also called Van Gogh-like protein 2 is a transmembrane protein involved in the planar cell polarity (PCP) pathway. It has a mass of approximately 60 kDa. This protein is expressed in various tissues including the brain heart and kidneys. In terms of mechanical function VANGL2 acts at the cell membrane to regulate cellular orientation and movement playing a central role in processes where cells need to align in a uniform direction.

Biological function summary

VANGL2 interacts with several other proteins to form the PCP complex. This complex controls the orientation of cells within the plane of a tissue. VANGL2 regulates cytoskeletal dynamics and affects cell signaling influencing important cellular processes like migration and intercellular communication. Moreover its presence is essential for the proper development and functioning of various organ systems including the neural tube and cardiac outflow tract.

Pathways

VANGL2 appears in the PCP signaling and Wnt signaling pathways. These pathways are critical in shaping tissue architecture during embryonic development. In the PCP pathway VANGL2 interacts with proteins such as Dishevelled and Prickle. In the Wnt signaling pathway VANGL2 plays a part in forwarding signals that control cell fate decisions and proliferation connecting to proteins like Frizzled and Beta-catenin.

VANGL2 has strong ties to conditions such as neural tube defects and congenital heart disease. Mutations in VANGL2 can disrupt normal developmental processes leading to these disorders. The protein interacts with SCRIB in the context of neural tube defects where misregulation can result in conditions like spina bifida. Similarly VANGL2 mutations interfere with NOTCH signaling a pathway linked to congenital heart disease illustrating its significant role in maintaining cardiovascular health.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Required for cell surface localization of FZD3 and FZD6 in the inner ear (By similarity).

See full target information VANGL2

대체 명칭 보기

KIAA1215, STB1, VANGL2, Vang-like protein 2, Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Glia 68:1840-1858 PubMed32125730

2020

The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew A Jarjour,Atanaska N Velichkova,Amanda Boyd,Kathryn M Lord,Carole Torsney,Deborah J Henderson,Charles Ffrench-Constant

Molecular medicine reports 11:851-6 PubMed25352048

2014

miR‑542‑3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh‑like 2.

Applications

Species

Human

Huazhuang Li,Hongtao Liu,Jingfang Pei,Haiyan Wang,Honglin Lv

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VANGL2 antibody

Western blot - Anti-VANGL2 antibody (AB60172)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise