Anti-VAP1 antibody

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody (AB42885)

IHC image of VAP1 antibody staining in a section of formalin-fixed paraffin-embedded normal human lung* performed on a Leica BOND^TM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab42885, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Project

Western blot - Anti-VAP1 antibody (AB42885)

VAP1 has a predicted molecular weight of 85 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). The observed 100kDa band is consistent with other commercially avaliable antibodies.

All lanes:

Western blot - Anti-VAP1 antibody (ab42885) at 1 µg/mL

Lane 1:

Lung (Rat) Tissue Lysate at 10 µg

Lane 2:

Lung (Mouse) Whole Cell Lysate - normal tissue at 10 µg

Lane 3:

Lung (Human) Tissue Lysate - Fetal Normal Tissue at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 85 kDa

Observed band size: 100 kDa,110 kDa

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