JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB244312

Anti-VAPA antibody

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Polyclonal VAPA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VAPA aa 100-300.

대체 명칭 보기

VAP33, VAPA, Vesicle-associated membrane protein-associated protein A, VAMP-A, VAMP-associated protein A, VAP-A, 33 kDa VAMP-associated protein, VAP-33

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-VAPA antibody (AB244312)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VAPA antibody (AB244312)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for VAPA (green) using ab244312 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (AB244312)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (AB244312)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for VAPA using ab244312 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-VAPA antibody (AB244312)
  • WB

Unknown

Western blot - Anti-VAPA antibody (AB244312)

All lanes:

Western blot - Anti-VAPA antibody (ab244312) at 0.4 µg/mL

All lanes:

MOLT-4 (human lymphoblastic leukemia cell line) cell lysate

Predicted band size: 28 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human VAPA aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q9P0L0

style
left-column

대체품

Primary Antibodies

AB225890

Anti-VAPA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (AB225890)

  • 0
  • 0
Primary Antibodies

AB181067

Anti-VAPA antibody [EPR13589(B)] - N-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

  • 5
  • 2
Primary Antibodies

AB96584

Anti-VAPA antibody

Western blot - Anti-VAPA antibody (AB96584)

  • 0
  • 0

보완 제품

Primary Antibodies

AB314225

Anti-RFP antibody [EPR28246-45]

BOND RX™ Validated|Recombinant|RabMAb|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody [EPR28246-45] (AB314225)

  • 1
  • 1
Primary Antibodies

AB15576

Anti-SEC61B antibody

Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB15576)

  • 4
  • 1
Assay Kits

AB133116

Cholesterol Assay Kit (Cell-Based)

Fluorescence Microscopy - Cholesterol Assay Kit (Cell-Based) (AB133116)

  • 5
  • 1
Primary Antibodies

AB218195

Anti-Phosphothreonine antibody [EPR22006-23]

RabMAb|Recombinant

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (AB218195)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein-associated protein A (VAPA) also known as VAP-A is an integral membrane protein involved in intracellular membrane trafficking. It has a molecular weight of approximately 33 kDa and is expressed in multiple tissues including the brain liver and muscles. Mechanically VAPA functions in the process of vesicle docking and fusion at membranes which is essential for the trafficking of proteins and lipids within cells. This protein is also a part of the endoplasmic reticulum (ER) and contributes to the formation of membrane contact sites between the ER and other organelles.
Biological function summary

In the cellular environment VAPA plays an important role in maintaining homeostasis by regulating lipid transport and metabolism. It forms a complex with several other proteins such as OSBP (oxysterol-binding protein) and CERT (ceramide transport protein) which facilitates the exchange of lipids between the ER and the Golgi apparatus. This interaction underlines VAPA’s role in lipid droplet dynamics and the synthesis of phosphatidylinositols which are critical for cell signaling and membrane integrity.

Pathways

The involvement of VAPA is significant in lipid metabolism and membrane trafficking pathways. It interacts with proteins like VAMP (vesicle-associated membrane proteins) and SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes which are important for exocytosis and endocytosis processes. VAPA's interaction in these pathways supports the movement of materials across cellular membranes and its regulation of lipid exchange impacts the signaling pathways influencing cell growth and differentiation.

Abnormal VAPA function relates to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and some forms of motor neuron disease. In such conditions VAPA’s interaction with proteins like TDP-43 (TAR DNA-binding protein 43) becomes disrupted leading to protein aggregation and neuronal toxicity. Additionally disruptions in VAPA-associated lipid transport are linked to metabolic disorders like type 2 diabetes highlighting its critical role in proper cellular communication and metabolic balance.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed : 32344433, PubMed : 33124732). STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes (PubMed : 33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed : 32344433). In addition, recruited to the plasma membrane through OSBPL3 binding (PubMed : 25447204). The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed : 25447204). With OSBPL3, may regulate ER morphology (PubMed : 16143324). May play a role in vesicle trafficking (PubMed : 11511104, PubMed : 19289470).
See full target information VAPA
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com