Anti-VAT1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-VAT1 antibody (AB242045)

VAT1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242045 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab242045 at 0.4 μg/ml.

Lane 1 : ab242045 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

Prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-VAT1 antibody (ab242045)

Predicted band size: 42 kDa

false

• WB

Supplier Data

Western blot - Anti-VAT1 antibody (AB242045)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-VAT1 antibody (ab242045) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 42 kDa

true

Exposure time: 10s