AB252999

Anti-VAT1 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal VAT1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VAT1 aa 100-200.

대체 명칭 보기

NADPH-dependent quinone oxidoreductase VAT1, Mitofusin-binding protein, Synaptic vesicle membrane protein VAT-1 homolog, Vesicle amine transport 1, MIB, VAT-1, VAT1

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAT1 antibody (AB252999)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAT1 antibody (AB252999)

Paraffin-embedded human urinary-bladder tissue stained for VAT1 using ab252999 at 1/200 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-VAT1 antibody (AB252999)

All lanes:

Western blot - Anti-VAT1 antibody (ab252999) at 0.4 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 42 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human VAT1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q99536

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

VAT1 also known as Vesicle Amine Transport Protein 1 homolog is a protein involved mechanically in the transport and storage of amines in synaptic vesicles. It has an approximate mass of 45 kDa. VAT1 is highly expressed in the nervous system particularly in regions with a high density of synaptic vesicles. It localizes to the membrane of synaptic vesicles suggesting its role in vesicular trafficking and neurotransmitter transport processes.

Biological function summary

VAT1 functions in vesicular transport and neurotransmitter storage playing a role in synaptic transmission. This protein does not work alone; it contributes to a larger complex involved in establishing proper neurotransmitter concentrations within synaptic vesicles. VAT1 may also participate in modulating synaptic plasticity which influences learning and memory processes. Its activity ensures proper nerve signal transmission across synapses by maintaining neurotransmitter levels.

Pathways

The VAT1 protein is important in neurotransmitter release and recycling pathways in the nervous system. It associates with proteins like synaptophysin and synapsin I to facilitate vesicle docking and release. VAT1 plays a role in the synaptic vesicle cycle which includes vesicle loading exocytosis and recycling. Its function guarantees that neurotransmitters are quickly replenished promoting efficient synaptic communication.

VAT1 associates with neurological disorders such as Parkinson’s disease and epilepsy where neurotransmitter imbalances are evident. Connections between VAT1 and the protein alpha-synuclein are apparent in the context of Parkinson’s disease as disruptions in vesicular transport can lead to toxic protein aggregation. In epilepsy disturbances in VAT1 function can impact synaptic efficacy and neurotransmitter availability contributing to seizure activity. Understanding VAT1 function can provide insights into these disorders and offer targets for therapeutic intervention.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Multifunctional protein with enzymatic and transport activities (PubMed : 17105775, PubMed : 26394711, PubMed : 32005660, PubMed : 33483563). Probable NADPH-dependent quinone oxidoreductase which physiological substrates and function are unclear (PubMed : 33483563). Alternatively, has also been suggested to mediate the transfer of negatively charged phospholipids including phosphatidic acid (PA), phosphatidylserine (PS) and phosphatidylglycerol (PG) between endoplasmic reticulum and mitochondrial membranes (PubMed : 26394711, PubMed : 32005660). Could regulate mitochondrial fusion through its interaction with mitofusins (PubMed : 17105775).

See full target information VAT1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cell and developmental biology 10:836041 PubMed35281088

2022

iTRAQ-Based Proteome Profiling of Differentially Expressed Proteins in Insulin-Resistant Human Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yan,Bei Xie,Ye Tian,Li Huang,Shuli Zou,Zhiheng Peng,Zhuan Liu,Linjing Li

Neoplasma 68:416-422 PubMed33440991

2021

Expression of VAT1 in hepatocellular carcinoma and its clinical significance.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Heng,Fei Wei,Wanhai Li,Qian Li,Dan-Lei Xiong,Ying-Yu Ma,Da-Hong Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VAT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAT1 antibody (AB252999)

Western blot - Anti-VAT1 antibody (AB252999)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise