Anti-Vav proteins antibody [EP482Y]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (AB40875)

Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• WB

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Western blot - Anti-Vav proteins antibody [EP482Y] (AB40875)

All lanes:

Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Predicted band size: 98 kDa

Observed band size: 98 kDa

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