Anti-Vav proteins (phospho Y174) antibody

• WB

Unknown

Western blot - Anti-Vav proteins (phospho Y174) antibody (AB47282)

All lanes:

Western blot - Anti-Vav proteins (phospho Y174) antibody (ab47282) at 1/500 dilution

Lane 1:

Extrats from Jurkat cells treated with UV at 30 µg

Lane 2:

Extrats from Jurkat cells untreated at 30 µg

Predicted band size: 78 kDa

false

• WB

CiteAb

Western blot - Anti-Vav proteins (phospho Y174) antibody (AB47282)

Western Blotting using Anti-Vav proteins (phospho Y174) antibody, ab47282. Publication image from Booth, C. et al., 2016, Nat Commun, 27221592. Legend direct from paper.

Synergistic activation of NF-κB is Vav1-dependent.(a) Rested NKL cells were stimulated with NKG2D and/or 2B4 in combination with or without CD94 engagement by receptor crosslinking. Lysates were immunoblotted for pS536-p65, pS276-p65, p65, p-Akt, p-Erk1/2, pY174-Vav1 or actin. The normalized intensities of the phosphorylated p65 relative to p65 are presented. (b) Rested NKL-κB-GFP cells were stimulated with plate-immobilized NKG2D and 2B4 in combination with or without CD94 for 6 h. GFP expression in the reporter NKL cells was analysed by flow cytometry, and representative result (left) and statistical bar charts (right) are shown. Values represent mean±s.d. (c) Rested NKL cells transfected with control siRNA or Vav1-specific siRNA were stimulated with NKG2D and/or 2B4 by receptor crosslinking. Lysates were immunoblotted for pS536-p65, pS276-p65, p65, p-Akt, p-Erk1/2, Vav1 or actin. The normalized intensities of the phosphorylated p65 relative to p65 are presented. (d) Rested NKL-κB-GFP cells transfected with control siRNA or Vav1-specific siRNA were stimulated with plate-immobilized NKG2D and 2B4 for 6 h. GFP expression in the reporter NKL cells was analysed by flow cytometry, and representative result (left) and statistical bar charts (right) are shown. Values represent mean±s.d. **P<0.01; ***P<0.001 (two-sided Student's t-test). Data are representative of at least three independent experiments.

false

• WB

CiteAb

Western blot - Anti-Vav proteins (phospho Y174) antibody (AB47282)

Western Blotting using Anti-Vav proteins (phospho Y174) antibody, ab47282. Publication image from Booth, C. et al., 2016, Nat Commun, 27221592. Legend direct from paper.

Defective cytotoxic degranulation and Vav1 activation in XLP1 NK cells following coactivation.(a,b) Primary rested NK cells after expansion from normal or XLP1 donors were mixed with P815 target cells as indicated in the presence of fluorochrome-conjugated anti-CD107a mAb. After incubation for 2 h, cells were analysed using flow cytometry as described in Fig. 5b. (a) Representative result is shown. (b) Percent increase of CD107a+ NK cells obtained from individual normal or XLP1 donors after stimulation with the indicated receptors relative to CD107a+ NK cells without stimulation (δCD107a+ cells). Values represent mean±s.d. (c) Representative FACS profiles showing the expression levels of the NKp30, NKp44, NKp46, NKG2D, NKG2C, DNAM-1, 2B4 and CD16 receptors (shaded histogram) on primary expanded NK cells obtained from normal or XLP1 donors. Isotype control staining is shown as the solid lines. (d) Primary rested NK cells after expansion from normal or XLP1 patient donors were treated as in Fig. 5d to stimulate NKG2D and/or 2B4 for 2 min. Lysates were immunoblotted with anti-pY174-Vav1 Ab and reprobed for Vav1. The normalized intensities of the phosphorylated Vav1 relative to total Vav1 are presented. Representative result (left) and statistical bar charts for pooled data from three different donors (right) are shown. Values represent mean±s.e.m. *P<0.05 (two-sided Student's t-test).

false

• WB

CiteAb

Western blot - Anti-Vav proteins (phospho Y174) antibody (AB47282)

Western Blotting using Anti-Vav proteins (phospho Y174) antibody, ab47282. Publication image from Booth, C. et al., 2016, Nat Commun, 27221592. Legend direct from paper.

Synergistic activation of NF-κB is Vav1-dependent.(a) Rested NKL cells were stimulated with NKG2D and/or 2B4 in combination with or without CD94 engagement by receptor crosslinking. Lysates were immunoblotted for pS536-p65, pS276-p65, p65, p-Akt, p-Erk1/2, pY174-Vav1 or actin. The normalized intensities of the phosphorylated p65 relative to p65 are presented. (b) Rested NKL-κB-GFP cells were stimulated with plate-immobilized NKG2D and 2B4 in combination with or without CD94 for 6 h. GFP expression in the reporter NKL cells was analysed by flow cytometry, and representative result (left) and statistical bar charts (right) are shown. Values represent mean±s.d. (c) Rested NKL cells transfected with control siRNA or Vav1-specific siRNA were stimulated with NKG2D and/or 2B4 by receptor crosslinking. Lysates were immunoblotted for pS536-p65, pS276-p65, p65, p-Akt, p-Erk1/2, Vav1 or actin. The normalized intensities of the phosphorylated p65 relative to p65 are presented. (d) Rested NKL-κB-GFP cells transfected with control siRNA or Vav1-specific siRNA were stimulated with plate-immobilized NKG2D and 2B4 for 6 h. GFP expression in the reporter NKL cells was analysed by flow cytometry, and representative result (left) and statistical bar charts (right) are shown. Values represent mean±s.d. **P<0.01; ***P<0.001 (two-sided Student's t-test). Data are representative of at least three independent experiments.

false