AB76225

Anti-VAV1 (phospho Y174) antibody [EP510Y]

Name: Anti-VAV1 (phospho Y174) antibody [EP510Y]
Brand: Abcam Limited
SKU: AB76225
Rating: 5 (1 reviews)

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(20 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal VAV phospho Y174 antibody. Suitable for Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide, Human samples. Cited in 20 publications.

4 이미지

Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) untreated/treated with 1mM pervanadate for 30min cells labeling VAV1 with unpurified ab76225 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control (Green).

Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Immunocytochemistry/Immunofluorescence analysis of Untreated Jurkat cells and Pervanadate (1mM,30min) treated Jurkat cells labelling VAV1 (phospho Y174) with purified ab76225 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, and counterstained with ab7291 anti-Tubulin (mouse mAb) 1 : 1000 (1ug/ml). An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Negative Control 1 : Rabbit primary antibody and anti-mouse secondary antibody(ab150120)

Negative Control 2 : Mouse primary antibody(ab7291) and anti-rabbit secondary antibody(ab150077)

Lab

Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

Lane 2:

Jurkat treated with 50mM pervanadate for 5minutes whole cell lysate at 10 µg

Lane 3:

Jurkat treated with 50mM pervanadate for 5min whole cell lysate 10 μg. Then the membrane was incubated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 98 kDa

false

Unknown

Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Dot blot analysis of VAV1 (pY174) peptide (Lane 1) and VAV1 non-phospho peptide (Lane 2) labelling VAV1 (phospho Y174) with ab76225 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP510Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), Dot, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

VAV1 also known as VAV-1 is a guanine nucleotide exchange factor with a mass of approximately 95 kDa. It activates Rho family GTPases by promoting the exchange of GDP for GTP. VAV1 is mainly expressed in hematopoietic cells playing an important role in immune responses. Its structure includes several domains – Dbl homology pleckstrin homology and an SH3 domain – that contribute to its function by facilitating interactions with other proteins.

Biological function summary

VAV1 regulates the signaling processes that lead to actin cytoskeletal rearrangements cell proliferation and differentiation. It forms part of larger multiprotein complexes that transmit signals from the cell surface to the nucleus. The activity of VAV1 is important for normal lymphocyte development and activation. By interacting with surface receptors it influences downstream effects altering cellular behavior and promoting adaptive immune responses.

Pathways

VAV1 is integral to the T cell receptor (TCR) signaling pathway and the B cell receptor (BCR) pathway. It acts as a link between the receptors and the Rho family of GTPases such as Rac1 and Cdc42 mediating the transmission of signals that result in cellular responses. The coupling of VAV1 with proteins like LAT and SLP-76 strengthens the signaling cascade necessary for lymphocyte activation and proliferation.

Deregulation of VAV1 is associated with certain cancers such as leukemia and lymphoma. Changes in VAV1 expression or activity can disrupt normal immune cell signaling promoting unchecked growth and survival of malignant cells. Additionally VAV1 is linked to autoimmune diseases where its altered function can lead to inappropriate immune responses. VAV1 interactions with proteins like ZAP-70 highlight its role in these pathologies highlighting its significance as a potential therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

See full target information VAV1 pY174

대체 명칭 보기

VAV, VAV1, Proto-oncogene vav

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제품이 사용된 논문 (20)

Recent publications for all applications. Explore the 전체 목록and refine your search

Theranostics 15:9344-9358 PubMed41280875

2025

Natural killer cell-specific chimeric antigen receptor enhances CAR NK cell functions and anti-tumor activity.

Applications

Species

Human

Changqing Pan,You Zhai,Zhongliang Cui,Yiyun Yin,Menghui Xu,Di Wang,Yishuo Sun,Jiazheng Zhang,Chen Wang,Ziwei Li,Mingchen Yu,Zhongfang Shi,Guanzhang Li,Tao Jiang,Wei Zhang

Cell death & disease 15:332 PubMed38740744

2024

FOXP4-mediated induction of PTK7 activates the Wnt/β-catenin pathway and promotes ovarian cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ji,Qilan Qian,Wenhao Cheng,Xiaoqing Ye,Aixin Jing,Shaojie Ma,Yuanyuan Ding,Xinhui Ma,Yasong Wang,Qian Sun,Xiujun Wang,Yulu Chen,Lan Zhu,Qing Yuan,Menghan Xu,Jingting Qin,Lin Ma,Jiayan Yang,Meiqi Zhang,Ting Geng,Sen Wang,Dan Wang,Yizhuo Song,Boyu Zhang,Yuting Xu,Linyu Xu,Shunfang Liu,Wei Liu,Bin Liu

International immunopharmacology 119:110177 PubMed37068336

2023

Esculin alleviates LPS-induced acute lung injury via inhibiting neutrophil recruitment and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangwei Ni,Ge Li,Ningfeng Dai,Zijiao Quan,Haibin Tong,Yu Liu

Cell reports 39:110695 PubMed35443168

2022

Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jose R Cortes,Ioan Filip,Robert Albero,Juan A Patiño-Galindo,S Aidan Quinn,Wen-Hsuan W Lin,Anouchka P Laurent,Bobby B Shih,Jessie A Brown,Anisha J Cooke,Adam Mackey,Jonah Einson,Sakellarios Zairis,Alfredo Rivas-Delgado,Maria Antonella Laginestra,Stefano Pileri,Elias Campo,Govind Bhagat,Adolfo A Ferrando,Raul Rabadan,Teresa Palomero

Frontiers in medicine 8:675963 PubMed34414199

2021

Downregulation of CD45 Signaling in COVID-19 Patients Is Reversed by C24D, a Novel CD45 Targeting Peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Danny Alon,Yossi Paitan,Eyal Robinson,Nirit Ganor,Julia Lipovetsky,Rinat Yerushalmi,Cyrille J Cohen,Annat Raiter

Oncoimmunology 10:1929725 PubMed34104545

2021

A novel role for an old target: CD45 for breast cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Annat Raiter,Oran Zlotnik,Julia Lipovetsky,Shany Mugami,Shira Dar,Ido Lubin,Eran Sharon,Cyrille J Cohen,Rinat Yerushalmi

The Journal of clinical investigation 130:3087-3097 PubMed32069268

2020

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Guedan,Aviv Madar,Victoria Casado-Medrano,Carolyn Shaw,Anna Wing,Fang Liu,Regina M Young,Carl H June,Avery D Posey

Cellular & molecular immunology 16:897-907 PubMed31316154

2019

Thymic-specific regulation of TCR signaling by Tespa1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Lyu,Pengfei Wang,Ting Xu,Yichao Shen,Zejin Cui,Mingzhu Zheng,Guo Fu,Linrong Lu

Cancer letters 455:1-13 PubMed30981760

2019

CD100-plexin-B1 induces epithelial-mesenchymal transition of head and neck squamous cell carcinoma and promotes metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Hongjiang Qiao,Weinan Guo,Yuan Liu,Luting Yang,Yufeng Liu,Boquan Jin,Meng Fu,Gang Wang,Wei Li

BMC immunology 20:7 PubMed30674283

2019

CEACAM1 regulates the IL-6 mediated fever response to LPS through the RP105 receptor in murine monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifang Zhang,Deirdre La Placa,Tung Nguyen,Maciej Kujawski,Keith Le,Lin Li,John E Shively

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VAV1 (phospho Y174) antibody [EP510Y]

Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (20)

Product promise