Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (AB238424)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) untreated/treated with 1mM pervanadate for 30min cells labeling VAV1 with unpurified ab76225 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control (Green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76225).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (AB238424)

Immunocytochemistry/Immunofluorescence analysis of untreated Jurkat cells and Pervanadate (1 mM, 30 minutes) treated Jurkat cells labeling VAV1 (phospho Y174) with purified ab76225 at 1/500 dilution.

Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, and counterstained with ab7291 anti-Tubulin (mouse mAb) 1 : 1000 (1 μg/ml). An Alexa-Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Negative Control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120).

Negative Control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077).

This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, tissue culture supernatant, BSA, glycerol and sodium azide (ab76225).

• Dot

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Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (AB238424)

Dot blot analysis of VAV1 (pY174) peptide (Lane 1) and VAV1 non-phospho peptide (Lane 2) labelling VAV1 (phospho Y174) with ab76225 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76225).