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AB50899

Anti-VAX2 antibody [VAX2A8F12]

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Mouse Monoclonal VAX2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VAX2.
3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-VAX2 antibody [VAX2A8F12] (AB50899)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VAX2 antibody [VAX2A8F12] (AB50899)

ab50899 at 1/10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1/200 dilution.

Western blot - Anti-VAX2 antibody [VAX2A8F12] (AB50899)
  • WB

Unknown

Western blot - Anti-VAX2 antibody [VAX2A8F12] (AB50899)

All lanes:

Western blot - Anti-VAX2 antibody [VAX2A8F12] (ab50899) at 1/50 dilution

All lanes:

NIH3T3 whole cell lysate at 50 µg/mL

Secondary

All lanes:

Anti mouse IgG antibody at 1/2500 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

false

Western blot - Anti-VAX2 antibody [VAX2A8F12] (AB50899)
  • WB

Unknown

Western blot - Anti-VAX2 antibody [VAX2A8F12] (AB50899)

All lanes:

Western blot - Anti-VAX2 antibody [VAX2A8F12] (ab50899) at 1/50 dilution

All lanes:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

Anti mouse IgG antibody at 1/2500 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

VAX2A8F12

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human VAX2. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB154855

Anti-VAX2 antibody [EPR10424]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-VAX2 antibody [EPR10424] (AB154855)

  • 1
  • 1

보완 제품

Primary Antibodies

AB311130

Alexa Fluor® 647 Anti-PHOX2B antibody [EPR14423] - C-terminal

RabMAb|Recombinant

  • 0
  • 0
Primary Antibodies

AB52492

Anti-ALDH1A1 antibody [EP1933Y] - C-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (AB52492)

  • 4
  • 22
Assay Kits

AB113851

DCFDA / H2DCFDA - Cellular ROS Assay Kit

Functional Studies (Act) - DCFDA / H2DCFDA - Cellular ROS Assay Kit (AB113851)

  • 5
  • 11
Primary Antibodies

AB155279

Anti-SOX10 antibody [EPR4007]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (AB155279)

  • 4
  • 7
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Filtered through a 0.22 µm membrane
보관 버퍼
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

VAX2 also known as Ventral Anterior Homeobox 2 is a transcription factor with a significant role in embryonic development. It has a molecular mass of around 36 kDa. VAX2 is primarily expressed in the developing central nervous system including the brain and eyes. This protein belongs to the homeobox gene family which regulates patterns of anatomical development.
Biological function summary

VAX2 controls the expression of genes involved in eye development. It is part of a transcriptional complex that includes proteins like VAX1 and PAX6. Together these proteins coordinate the formation of structures within the eye such as the retina. VAX2 ensures the correct spatial patterning and differentiation of photoreceptor cells maintaining proper eye morphogenesis.

Pathways

VAX2 plays a role in the retinal morphogenesis pathway and interacts closely with the Hedgehog signaling pathway. It works in tandem with proteins such as Sonic Hedgehog (SHH) to influence cellular differentiation and positional identity during development. VAX2's expression is regulated by these pathways demonstrating its integral function in developmental processes.

VAX2 has connections to eye disorders like coloboma and microphthalmia. These conditions are linked to mutations or dysregulations in genes that work alongside VAX2 such as PAX6. Any disruptions in VAX2 expression or function can lead to improper eye development potentially resulting in visual impairment or blindness. VAX2’s role highlights its significance in maintaining normal developmental processes and preventing congenital disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription factor that may function in dorsoventral specification of the forebrain. Regulates the expression of Wnt signaling antagonists including the expression of a truncated TCF7L2 isoform that cannot bind CTNNB1 and acts therefore as a potent dominant-negative Wnt antagonist. Plays a crucial role in eye development and, in particular, in the specification of the ventral optic vesicle (By similarity). May be a regulator of axial polarization in the retina.
See full target information VAX2

대체 명칭 보기

Ventral anterior homeobox 2, VAX2
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 292:2881-2892 PubMed28049726

2017

Biochemical Evidence for a Nuclear Modifier Allele (A10S) in TRMU (Methylaminomethyl-2-thiouridylate-methyltransferase) Related to Mitochondrial tRNA Modification in the Phenotypic Manifestation of Deafness-associated 12S rRNA Mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Feilong Meng,Xiaohui Cang,Yanyan Peng,Ronghua Li,Zhengyue Zhang,Fushan Li,Qingqing Fan,Anna S Guan,Nathan Fischel-Ghosian,Xiaoli Zhao,Min-Xin Guan
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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