AB216035

Anti-VCAM1 antibody [VCAM1/843]

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Mouse Monoclonal VCAM1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VCAM1.

대체 명칭 보기

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [VCAM1/843] (AB216035)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody [VCAM1/843] (AB216035)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue labeling VCAM1 with ab216035 at 1 μg/ml.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

VCAM1/843

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VCAM1.

P19320

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대체품

Primary Antibodies

AB223982

FITC Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - FITC Anti-VCAM1 antibody [EPR5047] (AB223982)

Primary Antibodies

AB195540

HRP Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-VCAM1 antibody [EPR5047] (AB195540)

Primary Antibodies

AB223981

PE Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-VCAM1 antibody [EPR5047] (AB223981)

Primary Antibodies

AB215380

Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (AB215380)

Primary Antibodies

AB194319

Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (AB194319)

Primary Antibodies

AB174279

Anti-VCAM1 antibody [EPR5038(2)]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

보완 제품

Assay Kits

AB223591

Human VCAM1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human VCAM1 ELISA Kit (AB223591)

Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

Primary Antibodies

AB282575

Anti-ICAM1 antibody [EPR24639-3]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-ICAM1 antibody [EPR24639-3] (AB282575)

Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

Primary Antibodies

AB318152

Anti-VE Cadherin antibody [RM2022]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [RM2022] (AB318152)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

ab216035 was purified from Bioreactor Concentrate by Protein A/G.

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).

See full target information VCAM1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of experimental medicine 218: PubMed33779682

2021

Inhibition of macrophage histone demethylase JMJD3 protects against abdominal aortic aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Frank M Davis,Lam C Tsoi,William J Melvin,Aaron denDekker,Rachael Wasikowski,Amrita D Joshi,Sonya Wolf,Andrea T Obi,Allison C Billi,Xianying Xing,Christopher Audu,Bethany B Moore,Steven L Kunkel,Alan Daugherty,Hong S Lu,Johann E Gudjonsson,Katherine A Gallagher

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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