Anti-VEGF Receptor 2 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody (AB39638)

ICC/IF image of ab39638 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39638, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemical analysis of human brain tissue stained with ab39638. The image on the right is blocked with the synthesized peptide.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemical analysis of human stomach cancer tissue stained with ab39638 at 1/200 dilution at 4^oC overnight. The negative control was secondary antibody only.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB39638)

Immunohistochemical analysis of rat spinal cord tissue stained with ab39638 at 1/200 dilution at 4^oC overnight. The negative control was secondary antibody only.

• WB

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Western blot - Anti-VEGF Receptor 2 antibody (AB39638)

Western blot analysis of extracts from SK-OV3 cells using VEGF Receptor 2 antibody ab39638 (Lane 1 and 2) and VEGF Receptor 2 (phospho-Tyr951) antibody (Lane 3 and 4).

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-VEGF Receptor 2 antibody (ab39638)

Predicted band size: 151 kDa

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