AB209835

Anti-VEGFA antibody

Lab Essentials
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(2 제품이 사용된 논문 )

Rabbit Polyclonal VEGFA antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish vegfaa aa 1-200.

2 이미지

IHC - Wholemount - Anti-VEGFA antibody (AB209835)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-VEGFA antibody (AB209835)

Immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling VEGFA with ab209835 at 1/200.

IHC - Wholemount - Anti-VEGFA antibody (AB209835)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-VEGFA antibody (AB209835)

Immunohistochemical analysis of paraformaldehyde-fixed zebrafish embryos labeling VEGFA with ab209835 at 1/200.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Zebrafish

Applications

IHC - Wmt

Immunogen

Recombinant Fragment Protein within Zebrafish vegfaa aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB213244

Anti-VEGFA antibody [EPR21219]

BOND RX™ Validated|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EPR21219] (AB213244)

0
2

Primary Antibodies

AB209439

PE Anti-VEGFA antibody [EP1176Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - PE Anti-VEGFA antibody [EP1176Y] (AB209439)

0
0

Primary Antibodies

AB206888

Alexa Fluor® 594 Anti-VEGFA antibody [EP1176Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-VEGFA antibody [EP1176Y] (AB206888)

0
0

Primary Antibodies

AB206887

Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-VEGFA antibody [EP1176Y] (AB206887)

0
0

Primary Antibodies

AB185238

Anti-VEGFA antibody [EP1176Y] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody [EP1176Y] - BSA and Azide free (AB185238)

5
1

Primary Antibodies

AB206886

Alexa Fluor® 488 Anti-VEGFA antibody [EP1176Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-VEGFA antibody [EP1176Y] (AB206886)

0
0

Primary Antibodies

AB214424

Anti-VEGFA antibody [EPR20705]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-VEGFA antibody [EPR20705] (AB214424)

0
0

Primary Antibodies

AB52917

Anti-VEGFA antibody [EP1176Y] - C-terminal

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EP1176Y] - C-terminal (AB52917)

5
9

보완 제품

Primary Antibodies

AB214424

Anti-VEGFA antibody [EPR20705]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-VEGFA antibody [EPR20705] (AB214424)

0
0

Primary Antibodies

AB209710

Anti-VASA/VAS antibody

IHC - Wholemount - Anti-VASA/VAS antibody (AB209710)

0
0

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

5
104

Primary Antibodies

AB211143

Anti-THBS4 antibody - N-terminal

IHC - Wholemount - Anti-THBS4 antibody - N-terminal (AB211143)

5
1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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제품 세부 정보

Please refer to the vial label for the specific concentration.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.

Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Required for intersegmental vessel development in the tail during embryogenesis (PubMed : 15305301). Acts both upstream of kdr and tie1 to stimulate endothelial cell differentiation, and upstream of gata1 to stimulate hematopoietic cell differentiation.

See full target information vegfaa

대체 명칭 보기

vegf, vegfa, vegfaa, Vascular endothelial growth factor A-A, VEGF-A-A

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 275:111340 PubMed36347467

2022

Cardiovascular oxygen transport and peripheral oxygen extraction capacity contribute to acute heat tolerance in European seabass.

Applications

Unspecified application

Species

Unspecified reactive species

Katja Anttila,Florian Mauduit,Mirella Kanerva,Miriam Götting,Mikko Nikinmaa,Guy Claireaux

Frontiers in oncology 10:162 PubMed32133296

2020

Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Engqvist,Toshima Z Parris,Anikó Kovács,Elisabeth Werner Rönnerman,Karin Sundfeldt,Per Karlsson,Khalil Helou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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