JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB240200

Anti-Versican antibody [EPR12277] - BSA and Azide free

4

(1 리뷰)

|

(1 출판물)

Rabbit Recombinant Monoclonal Versican antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
2 이미지
Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200)
  • WB

Unknown

Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200)

Blocking and diluting buffer : 5% NFDM/TBST

This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID : 27683612, 24401530, 29222454, 19662655)

This data was developed using ab177480, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Versican antibody [EPR12277] (<a href='/ko/products/primary-antibodies/versican-antibody-epr12277-ab177480'>ab177480</a>) at 0.1 µg/mL

Lane 1:

Human hippocampus lysate at 20 µg

Lane 2:

Mouse hippocampus lysates at 20 µg

Lane 3:

Mouse heart lysates at 20 µg

Lane 4:

Rat hippocampus lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 373 kDa

false

Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200)
  • WB

Supplier Data

Western blot - Anti-Versican antibody [EPR12277] - BSA and Azide free (AB240200)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177480).

All lanes:

Western blot - Anti-Versican antibody [EPR12277] (<a href='/ko/products/primary-antibodies/versican-antibody-epr12277-ab177480'>ab177480</a>) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Fetal heart lysate at 10 µg

Predicted band size: 373 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12277

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

대체품

Primary Antibodies

AB171887

Anti-Versican antibody

  • 0
  • 0
Primary Antibodies

AB270445

Anti-Versican antibody [EPR23374-151]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23374-151] (AB270445)

  • 0
  • 0
Primary Antibodies

AB272178

Anti-Versican antibody [EPR23135-58] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23135-58] - BSA and Azide free (AB272178)

  • 0
  • 0
Primary Antibodies

AB269270

Anti-Versican antibody [EPR23135-58]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23135-58] (AB269270)

  • 0
  • 0
Primary Antibodies

AB272223

Anti-Versican antibody [EPR23374-21] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Versican antibody [EPR23374-21] - BSA and Azide free (AB272223)

  • 0
  • 0
Primary Antibodies

AB272224

Anti-Versican antibody [EPR23374-151] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23374-151] - BSA and Azide free (AB272224)

  • 0
  • 0
Primary Antibodies

AB19345

Anti-Versican antibody

  • 5
  • 4
Primary Antibodies

AB270444

Anti-Versican antibody [EPR23374-21]

RabMAb|Recombinant

Western blot - Anti-Versican antibody [EPR23374-21] (AB270444)

  • 0
  • 0

보완 제품

Assay Kits

AB283883

Human Versican ELISA Kit

Recombinant|SimpleStep

ELISA - Human Versican ELISA Kit (AB283883)

  • 0
  • 0
Sample Prep & Detection Kits

AB269901

Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link®

Schematic Diagram - Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® (AB269901)

  • 0
  • 0
Sample Prep & Detection Kits

AB269893

Latex Conjugation Kit - 400nm Red

Functional Studies - Latex Conjugation Kit - 400nm Red (AB269893)

  • 0
  • 0
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB6586

Anti-Collagen IV antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

  • 5
  • 123
Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

ab240200 is the carrier-free version of ab177480.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
style
left-column
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
See full target information VCAN

대체 명칭 보기

CSPG2, VCAN, Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, PG-M, Chondroitin sulfate proteoglycan 2, GHAP
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of oncology 54:77-86 PubMed30431069

2018

Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Song,Rui Wang,Li-Wei Li,Xi Liu,Yun-Fei Wang,Qi-Xue Wang,Qingyu Zhang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com