AB242204

Anti-VGluT1 antibody [CL2754]

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(3 제품이 사용된 논문 )

Mouse Monoclonal VGluT1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC17A7 aa 250-300.

대체 명칭 보기

BNPI, VGLUT1, SLC17A7, Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for VGluT1 with ab242204 at a 1 : 5000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Negative control : Formalin-fixed, paraffin-embedded human tonsil tissue stained for VGluT1 with ab242204 at a 1 : 5000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Formalin-fixed, paraffin-embedded rat brain tissue stained for VGluT1 with ab242204 at a 1 : 5000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Formalin-fixed, paraffin-embedded rat brain tissue stained for VGluT1 with ab242204 at a 1 : 5000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Formalin-fixed, paraffin-embedded mouse brain tissue stained for VGluT1 with ab242204 at a 1 : 5000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Formalin-fixed, paraffin-embedded mouse hippocampus tissue stained for VGluT1 with ab242204 at a 1 : 5000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-VGluT1 antibody [CL2754] (AB242204)

All lanes:

Western blot - Anti-VGluT1 antibody [CL2754] (ab242204) at 1 µg/mL

All lanes:

Human cerebral cortex tissue lysate

Predicted band size: 61 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2754

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC17A7 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q9P2U7

Epitope

Binds to an epitope located within the peptide sequence EERKYIEDAI as determined by overlapping synthetic peptides.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

VGluT1 or Vesicular Glutamate Transporter 1 is an important protein for transporting glutamate in the brain. It plays an important role in packaging glutamate into synaptic vesicles. This protein has an approximate mass of 61 kDa. Scientists find VGluT1 expression mainly in the central nervous system especially in areas involved in synaptic transmission and neural communication. VGluT1 localizes at the presynaptic terminals and enables glutamate to be stored in vesicles before release into the synaptic cleft.

Biological function summary

The function of VGluT1 centers on neurotransmitter regulation maintaining adequate synaptic glutamate levels. It does not work alone but works as part of a complex within the presynaptic vesicular organelles. This transport system enables efficient glutamate signaling essential for learning and memory. Through its action it supports important processes in brain development and synaptic plasticity indicating its role in cognitive functions.

Pathways

VGluT1 is essential in the glutamatergic signaling pathways which influence numerous neurotransmission activities. Its activity interlinks with proteins like synaptophysin and synapsins that help dock and release synaptic vesicles. Another associated pathway involves glutamate synthesis and recycling helping maintain excitatory neurotransmission balance. These pathways implicate VGluT1 in maintaining proper neural network connectivity and excitatory-inhibitory balance.

Altered VGluT1 function impacts conditions such as schizophrenia and amyotrophic lateral sclerosis (ALS). Researchers associate its dysregulation with impaired glutamate homeostasis leading to synaptic dysfunction. VGluT1 interacts with neuronal proteins such as EAAT2 involved in ALS where its malfunction results in motor neuron degeneration. In the context of schizophrenia altered VGluT1 expression disturbs glutamatergic neurotransmission contributing to symptoms due to synaptic imbalance.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed : 10820226). At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (By similarity). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (By similarity). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (By similarity). The vesicular K(+)/H(+) antiport activity is electroneutral (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (PubMed : 10820226). The symporter activity is driven by an inside negative membrane potential and is electrogenic (By similarity). Is necessary for synaptic signaling of visual-evoked responses from photoreceptors (By similarity).

See full target information SLC17A7

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Toxicological sciences : an official journal of the Society of Toxicology 201:48-60 PubMed38830033

2024

Developmental PFOS exposure alters lung inflammation and barrier integrity in juvenile mice.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph H Lucas,Qixin Wang,Jiries Meehan-Atrash,Cortney Pang,Irfan Rahman

Communications biology 6:1211 PubMed38017066

2023

Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline E Strong,Jiajing Zhang,Martin Carrasco,Srikanya Kundu,Molly Boutin,Harshad D Vishwasrao,Jiamin Liu,Angelica Medina,Yu-Chi Chen,Kelli Wilson,Emily M Lee,Marc Ferrer

Cerebral cortex (New York, N.Y. : 1991) 30:4064-4075 PubMed32163129

2020

Temporal Gating of Synaptic Competition in the Amygdala by Cannabinoid Receptor Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Natália Madeira,Ana Drumond,Rosalina Fonseca

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VGluT1 antibody [CL2754]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [CL2754] (AB242204)

Western blot - Anti-VGluT1 antibody [CL2754] (AB242204)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise