AB244291

Anti-Villin antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal Villin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1 aa 700 to C-terminus.

대체 명칭 보기

VIL, VIL1, Villin-1

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human kidney tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human small intestine tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human liver tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human gallbladder tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human tonsil tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Negative control.

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (AB244291)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (AB244291)

PFA-fixed, Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for Villin (green) using ab244291 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human pancreas tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

Paraffin-embedded human colon tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-Villin antibody (AB244291)

WB

Unknown

Western blot - Anti-Villin antibody (AB244291)

All lanes:

Western blot - Anti-Villin antibody (ab244291) at 0.4 µg/mL

Lane 1:

Caco-2 (human colorectal adenocarcinoma cell line) cell lysate

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) cell lysate

Predicted band size: 93 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

Immunogen

Recombinant Fragment Protein within Human VIL1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

style

left-column

대체품

Primary Antibodies

AB201989

Anti-Villin antibody [3E5G11] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)

4
5

Primary Antibodies

AB130751

Anti-Villin antibody [SP145]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Villin antibody [SP145] (AB130751)

5
5

Primary Antibodies

AB109516

Anti-Villin antibody [EPR3490]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (AB109516)

5
1

Primary Antibodies

AB133510

Anti-Villin antibody [EPR3491(3)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [EPR3491(3)] (AB133510)

0
0

Primary Antibodies

AB245749

Anti-Villin antibody [SP145] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Villin antibody [SP145] - BSA and Azide free (AB245749)

0
0

보완 제품

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

5
133

Primary Antibodies

AB108508

Anti-Lysozyme antibody [EPR2994(2)]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (AB108508)

5
4

Primary Antibodies

AB272692

Anti-MUC2 antibody [EPR23479-47]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|Trial Size|Trial Size

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (AB272692)

5
7

Primary Antibodies

AB76126

Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker (AB76126)

5
17

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

See full target information VIL1

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:1313 PubMed39900921

2025

USP5 stabilizes YTHDF1 to control cancer immune surveillance through mTORC1-mediated phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Na Shao,Lei Xi,Yangfan Lv,Muhammad Idris,Lin Zhang,Ya Cao,Jingyi Xiang,Xi Xu,Belinda X Ong,Qiongyi Zhang,Xu Peng,Xiaoyan Yue,Feng Xu,Chungang Liu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com